IL-18/IL-1F4 Antibody (25-2G) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 25-2G
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-18/IL-1F4 Antibody (25-2G) [Unconjugated] Summary

• Immunogen: Human IL-18/IL-1F4
• Specificity: This antibody reacts with human IL-18 on Western blotting.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: This antibody is stable for one year from the date of purchase when stored at 4°C. After reconstitution, avoid repeated freezing and thawing. For storage, prepare appropriate aliquots and freeze at -20°C.
• Buffer: This antibody is in lyophilized form. After reconstitution, IgG concentration should be 1 mg/mL in PBS (pH 7.2)/1% sucrose. Contains no preservatives.
• Preservative: No Preservative
• Reconstitution Instructions: Prepare a stock solution by dissolving the lyophilized antibody with 100 μL of distilled water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (25-2G) [Unconjugated]

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma . Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
7. Okamura, H., et al. Nature 378, 88-91 (1995)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18/IL-1F4 Antibody (D043-3)(10)

Publications using D043-3

• Saha, A;Islam, MM;Kumar, R;Ismail, AM;Garcia, E;Gullapali, RR;Chodosh, J;Rajaiya, J; Virus and Cell Specific HMGB1 Secretion and Subepithelial Infiltrate Formation in Adenovirus Keratitis bioRxiv : the preprint server for biology 2025-01-07 [PMID: 39829903] (Western Blot, Human)
• J Saetang, N Roongsawan, S Sangkhatha, SP Voravuthik, N Sangkaew, N Prompat, T Srichana, V Tipmanee Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity PeerJ, 2022-07-05;10(0):e13626. 2022-07-05 [PMID: 35811828] (Western Blot, Human)
• Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells Mol Med Rep, 2017-08-11;0(0):. 2017-08-11 [PMID: 28849014] (Western Blot, Human)
• Mingfang Zhang, Yuanlin Qi, Hui Li, Jiajun Cui, Lu Dai, Jacqueline A. Frank et al. AIM2 inflammasome mediates Arsenic-induced secretion of IL-1 beta and IL-18 OncoImmunology 6/2/2016 [PMID: 27471628]
• Naveen K Somanna, Anthony J Valente, Maike Krenz, Kerry S McDonald, Yusuke Higashi, Makoto Noda et al. Histone deacetyltransferase inhibitors Trichostatin A and Mocetinostat differentially regulate MMP9, IL-18 and RECK expression, and attenuate Angiotensin II-induced cardiac fibroblast migration and proliferation Hypertension Research 10/1/2016 [PMID: 27278287]
• Naveen K. Somanna, Anthony J. Valente, Maike Krenz, William P. Fay, Patrice Delafontaine, Bysani Chandrasekar The Nox1/4 Dual Inhibitor GKT137831 or Nox4 Knockdown Inhibits Angiotensin-II-Induced Adult Mouse Cardiac Fibroblast Proliferation and Migration. AT1 Physically Associates With Nox4 Journal of Cellular Physiology 5/1/2016 [PMID: 26445208]
• Valente AJ, Yoshida T, Izadpanah R et al. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin. Cell Signal. 6/1/2013 [PMID: 23541442]
• Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration. Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86. 2009-06-26 [PMID: 19561311] (Western Blot, Human)
• Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23. Exp. Dermatol., 2004-12-01;13(12):764-72. 2004-12-01 [PMID: 15560760] (IHC-Fr, Human)
• Hessian PA, Highton J, Kean A, Sun CK, Chin M Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Arthritis Rheum., 2003-02-01;48(2):334-8. 2003-02-01 [PMID: 12571841] (IHC, Human)

Bioinformatics

• Gene Symbol: IL18