IL-18/IL-1F4 Antibody (125-2H) [Unconjugated]

• Reactivity: Hu
• Applications: IP, B/N, ELISA(Cap)
• Clone: 125-2H
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-18/IL-1F4 Antibody (125-2H) [Unconjugated] Summary

• Immunogen: Human IL-18/IL-1F4
• Specificity: This antibody reacts with human IL-18 on Immunoprecipitation.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation
  • Neutralization

Packaging, Storage & Formulations

• Storage: This antibody solution is stable for one year from the date of purchase when stored at -20°C.
• Buffer: 100 μg IgG in 100 μL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (125-2H) [Unconjugated]

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma . Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

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Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18/IL-1F4 Antibody (D044-3)(27)

Publications using D044-3

• Vijayashree Mysore, Xavier Cullere, Matthew L. Settles, Xinge Ji, Michael W. Kattan, Michaël Desjardins et al. Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens Med (N Y) 2021-09-01 [PMID: 34414383]
• Mikhail A. Gavrilin, Evan R. Prather, Alex D. Vompe, Christian C. McAndrew, Mark D. Wewers cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation The Journal of Immunology 2021-03-15 [PMID: 33568399]
• H Flament, M Rouland, L Beaudoin, A Toubal, L Bertrand, S Lebourgeoi, C Rousseau, P Soulard, Z Gouda, L Cagninacci, AC Monteiro, M Hurtado-Ne, S Luce, K Bailly, M Andrieu, B Saintpierr, F Letourneur, Y Jouan, M Si-Tahar, T Baranek, C Paget, C Boitard, A Vallet-Pic, JF Gautier, N Ajzenberg, B Terrier, F Pène, J Ghosn, X Lescure, Y Yazdanpana, B Visseaux, D Descamps, JF Timsit, RC Monteiro, A Lehuen Outcome of SARS-CoV-2 infection is linked to MAIT cell activation and cytotoxicity Nature Immunology, 2021-02-02;0(0):. 2/2/2021 [PMID: 33531712]
• LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4652-4660. 2019-11-01 [PMID: 31743940] (Neutralization, Human)
• S Suliman, M Murphy, M Musvosvi, A Gela, EW Meermeier, H Geldenhuys, C Hopley, A Toefy, N Bilek, A Veldsman, WA Hanekom, JL Johnson, WH Boom, G Obermoser, H Huang, M Hatherill, DM Lewinsohn, E Nemes, TJ Scriba MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria J. Immunol., 2019-10-14;0(0):. 2019-10-14 [PMID: 31611259] (Neutralization, Human)
• F Magnotti, L Lefeuvre, S Benezech, T Malsot, L Waeckel, A Martin, S Kerever, D Chirita, M Desjonquer, A Duquesne, M Gerfaud-Va, A Laurent, P Sève, MR Popoff, T Walzer, A Belot, Y Jamilloux, T Henry Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients EMBO Mol Med, 2019-10-07;0(0):e10547. 10/7/2019 [PMID: 31589380]
• X Zhang, D He, S Gao, Y Wei, L Wang Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization Mol Med Rep, 2019-06-06;20(2):1241-1249. 2019-06-06 [PMID: 31173233] (Cell Culture, Human)
• SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j J. Immunol., 2017-12-27;0(0):. 2017-12-27 [PMID: 29282306] (ELISA Development (Capture), Human)
• E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD Respir. Res., 2017-08-22;18(1):159. 2017-08-22 [PMID: 28830544] (ELISA Development (Capture), Human)
• LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton Production and regulation of interleukin-1 family cytokines at the materno-fetal interface Cytokine, 2017-07-13;0(0):. 2017-07-13 [PMID: 28712670] (ELISA Development (Capture), Human)
• J Liu, G Song, X Lin, X Pang, T Meng Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells Exp Ther Med, 2017-02-21;13(4):1447-1455. 2/21/2017 [PMID: 28413491]
• Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016-09-02;6(0):32320. 2016-09-02 [PMID: 27586092] (Flow Cytometry, Neutralization, Human)
• Bacillus Calmette-Gu�rin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses J Immunol, 2016-07-13;0(0):. 2016-07-13 [PMID: 27412415] (Neutralization, Human)
• Martin R Goodier Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection J Immunol, 2016-05-27;197(1):313-25. 2016-05-27 [PMID: 27233958] (Neutralization, Human)
• Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. J Immunol, 2015-07-31;195(5):2141-8. 2015-07-31 [PMID: 26232426] (ELISA Development (Capture), Human)
• Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus. J Immunol, 2015-03-02;194(8):3890-900. 2015-03-02 [PMID: 25732728] (Neutralization, Human)
• Hedl M, Zheng S, Abraham C The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways. J Immunol, 2014-05-19;192(12):5924-32. 2014-05-19 [PMID: 24842757] (Neutralization, Human)
• Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1. Cytokine, 2013-11-23;65(2):222-30. 2013-11-23 [PMID: 24275551] (ELISA Development (Detection), Human)
• Valente AJ, Yoshida T, Izadpanah R et al. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin. Cell Signal. 6/1/2013 [PMID: 23541442]
• Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration. Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86. 2009-06-26 [PMID: 19561311] (Neutralization, Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human)
• Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis. Arthritis Rheum., 2006-08-01;54(8):2402-14. 2006-08-01 [PMID: 16869003] (Neutralization, Human)
• Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes. J. Immunol., 2006-05-01;176(9):5284-92. 2006-05-01 [PMID: 16621994] (Neutralization, Human)
• Skurk T, Kolb H, Muller-Scholze S, Rohrig K, Hauner H, Herder C The proatherogenic cytokine interleukin-18 is secreted by human adipocytes. Eur. J. Endocrinol., 2005-06-01;152(6):863-8. 2005-06-01 [PMID: 15941925] (ICC, Human)
• Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction. J. Immunol., 2003-06-01;170(11):5571-7. 2003-06-01 [PMID: 12759435] (Functional Assay, Neutralization, Human)

FAQs for IL-18/IL-1F4 Antibody (D044-3). (Showing 1 - 1 of 1 FAQs).

1. I would like to enquire regarding the epitopr of 125-2H mAb. Do you know if it binds total, free or bound IL-18?
   • We have not mapped the eptiope of the antibody and so we cannot be sure where the antibody binds. It appears it will see all forms but I cannot be absolutely positive.Since the immunogen is:Immunogen Human IL-18/IL-1F4Specificity This antibody reacts with human IL-18 on Immunoprecipitation.

Bioinformatics

• Gene Symbol: IL18