IL-18/IL-1F4 Antibody (125-2H) [Unconjugated]


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Product Details

Reactivity HuSpecies Glossary
Applications IP, B/N, ELISA(Cap)

Order Details

IL-18/IL-1F4 Antibody (125-2H) [Unconjugated] Summary

Human IL-18/IL-1F4
This antibody reacts with human IL-18 on Immunoprecipitation.
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  • Immunoprecipitation
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • Neutralization
  • ELISA Standard (Matched Pair)
Positive Control
Bcl-2 Lysate (NBP2-64941)
Read Publications using
D044-3 in the following applications:

Packaging, Storage & Formulations

This antibody solution is stable for one year from the date of purchase when stored at -20°C.
100 μg IgG in 100 μL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
No Preservative


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (125-2H) [Unconjugated]

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18


Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.
  1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-18/IL-1F4 Antibody (D044-3)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 9 applications: Antibody Array Development, ELISA Capture, ELISA Microarray Development, ELISA detection, Flow, Functional Assay, ICC, Luminex Development, Neut.

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Antibody Array Development
ELISA Capture
ELISA Microarray Development
ELISA detection
Functional Assay
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using D044-3 Applications Species
SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j J. Immunol., 2017;0(0):. 2017 [PMID: 29282306] (ELISA Capture,, Human) ELISA Capture Human
E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD Respir. Res., 2017;18(1):159. 2017 [PMID: 28830544] (ELISA Capture,, Human) ELISA Capture Human
LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton Production and regulation of interleukin-1 family cytokines at the materno-fetal interface Cytokine, 2017;0(0):. 2017 [PMID: 28712670] (ELISA Capture,, Human) ELISA Capture Human
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016;6(0):32320. 2016 [PMID: 27586092] (Flow,, Human) Flow Human
Bacillus Calmette-Gu�rin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses J Immunol, 2016;0(0):. 2016 [PMID: 27412415] (Neut,, Human) Neut Human
Martin R Goodier Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection J Immunol, 2016;197(1):313-25. 2016 [PMID: 27233958] (Neut,, Human) Neut Human
Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. J Immunol, 2015;195(5):2141-8. 2015 [PMID: 26232426] (ELISA Capture,, Human) ELISA Capture Human
Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus. J Immunol, 2015;194(8):3890-900. 2015 [PMID: 25732728] (Neut,, Human) Neut Human
Hedl M, Zheng S, Abraham C The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways. J Immunol, 2014;192(12):5924-32. 2014 [PMID: 24842757] (Neut,, Human) Neut Human
Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1. Cytokine, 2013;65(2):222-30. 2013 [PMID: 24275551] (ELISA detection,, Human) ELISA detection Human
Show All 18 Publications.

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FAQs for IL-18/IL-1F4 Antibody (D044-3). (Showing 1 - 1 of 1 FAQs).

  1. I would like to enquire regarding the epitopr of 125-2H mAb. Do you know if it binds total, free or bound IL-18?
    • We have not mapped the eptiope of the antibody and so we cannot be sure where the antibody binds. It appears it will see all forms but I cannot be absolutely positive.Since the immunogen is:Immunogen Human IL-18/IL-1F4Specificity This antibody reacts with human IL-18 on Immunoprecipitation.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Diseases for IL-18/IL-1F4 Antibody (D044-3)

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Gene Symbol IL18