Mouse IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

ELISA: Mouse IL-18 BPa/IL18BP ELISA Kit (Colorimetric) [NBP3-06799] - Samples were spiked with high concentrations of Mouse IL-18 BPa/IL18BP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse IL-18 BPa/IL18BP ELISA Kit (Colorimetric) [NBP3-06799] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Mouse IL-18 BPa/IL18BP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse IL-18 BPa/IL18BP in samples. No significant cross-reactivity or interference between Mouse IL-18 BPa/IL18BP and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.67
• Intra-Assay: 5.58
• Spike Recovery: 83 - 110 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL18BP

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

• IL18 BPa
• IL-18 BPa
• IL18BP
• IL-18BP
• IL18BPa
• interleukin 18 binding protein
• interleukin-18-binding protein
• MC51L-53L-54L homolog gene product
• tadekinig-alfa

Background

IL-18BP which binds to IL-18, also known as interferon-gamma inducing factor (IGIF) which is a pro-inflammatory cytokine that belongs to the IL-1 family (1). IL-18 is a pleiotropic factor involved in the regulation of both innate and acquired immune responses, playing a key role in autoimmune, inflammatory, and infectious diseases (2). Interleukin-18 binding protein (IL18-BP) is an inhibitor of the pro-inflammatory cytokine IL18. The recombinant forms of IL18BP did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity (3). IL-18 plays an important role in host defense against microbial pathogens. Many poxviruses encode homologous IL-18BP that neutralizes IL-18 activity. Data show that IL-18BP prevents IL-18 from binding to IL-18R by interacting with three residues that are part of the binding site for IL-18Ralpha (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-18 BPa/IL18BP ELISA Kit (NBP3-06799). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL18BP