IL-17RD/SEF Antibody - BSA Free

Western Blot: IL-17 RD/SEF Antibody [NBP1-32307] - Sample(30 ug of whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:500.

Western Blot: IL-17 RD/SEF Antibody [NBP1-32307] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:2000

Western Blot: IL-17 RD/SEF Antibody [NBP1-32307] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IL-17RD/SEF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-17RD/SEF Antibody - BSA Free (NBP1-32307) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IL-17RD/SEF. The exact sequence is proprietary.
• Localization: Golgi apparatus membrane, Single-pass type I membrane protein, Cell membrane, Single-pass type I membrane protein, Isoform 4: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL17RD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for IL-17RD/SEF Antibody - BSA Free

• FLJ35755
• IL-17 RD
• IL17RD
• IL-17RD
• IL-17RDIL-17 receptor D
• IL17Rhom
• IL17RLM
• IL17RLMDKFZp434N1928
• interleukin 17 receptor D
• interleukin-17 receptor D
• Interleukin-17 receptor-like protein
• MGC133309
• Sef homolog
• SEF
• SEFhSef

Background

Fibroblast growth factors (FGFs; see MIM 603726) are secreted proteins involved in cellular proliferation, migration, differentiation, and survival. FGF activity is negatively regulated by members of the 'sprouty' family (e.g., SPRY1, MIM 602465). The SEF protein is a modulator of FGF signaling.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for IL-17RD/SEF Antibody (NBP1-32307). (Showing 1 - 1 of 1 FAQ).

1. We are interested in your antibody IL17RD Antibody (NBP1-32307). However, we have a question regarding it. There are two WB images on your website, where you show the detection of IL17RD. There are two WB images on your website, where you show the detection of IL17RD. On the first image you point to an approximately 80kDa band in HEK293T cells. Is this a transfected protein or the endogenous one? If this is the endogeous IL17RD, why is it 80kDa, and not approximately 120kDa, as it is expected from a glycosylated transmembrane IL17RD? Or is this the cytosolic isoform 4 of IL17RD? Could you clarify this for us?
   • In regards to your inquiry the HEK293T cells are transfected with the protein as an overexpression lysate. This product however is also tested on endogenous samples of 3T3 and Mouse brain. In those samples a band of similar size was also observed. Despite the glycosylation of this protein we detected it at approx. 79 kDa in our samples. The antibody should not be specific for just isoform 4. Since it is picking up at the lower molecular weight it may be detecting the cytosolic form with more prevalence than the membrane bound protein since whole cell preps were used and not just membrane preps.

Bioinformatics

• Gene Symbol: IL17RD