IL-17RC Antibody [Unconjugated]

K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL‑17 RC Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2269, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RC Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑17 RC
  Leu21-Ala454
  Accession # NP_116121
• Specificity: Detects human IL-17 RC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse IL-17 RC and 5% cross-reactivity with recombinant human (rh) IL-17 R, rhIL-17 RD, and rhIL‑17B R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17RC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RC Antibody [Unconjugated]

• FLJ95963
• FLJ96005
• IL-17 RC
• IL-17 receptor C
• IL-17 RL
• IL17F receptor
• IL17RC
• IL-17RC
• IL17Rhom
• IL17RL
• IL-17RL
• IL17-RL
• interleukin 17 receptor C
• interleukin-17 receptor C
• Interleukin-17 receptor homolog
• Interleukin-17 receptor-like protein
• MGC10763
• zcytoR14

Background

IL-17 receptor C (IL-17 RC; also known as IL-17 RL and IL-17 Rhom) is an 85-110 kDa member of the IL-17 receptor family. This is one of five families that currently comprise the cytokine receptor superfamily (1-6). At this time, there are five members within the IL-17 receptor family, and these are termed IL-17 RA, B, C, D, and E. Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17(A), while IL-17 RB is reported to bind IL‑17B and IL-17E (2, 4). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. These include endothelial cells (6, 7), chondrocytes and osteoblasts (8), breast and prostatic epithelium (6), and fibroblasts, plus renal tubular epithelium and skeletal muscle cells (8, 9). Full-length IL-17 RC is synthesized as a 791 amino acid (aa) precursor (10, 11). It contains a 20 aa signal sequence, a 518 aa extracellular domain (ECD) (aa 21‑538), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region. Although IL-17 RA has two fibrinogen-like regions in its ECD that contribute to its function, no such architecture has been identified in the ECD of IL-17 RC (12). Based on studies looking at exon deletions, a key ligand-binding site would appear to exist over aa 425-441 (13). The gene for human IL-17 RC contains 19 exons. It is estimated that there are over 90 alternative splice forms, with transmembrane‑containing isoforms predominating (6, 14). The full-length isoform is estimated to occur approximately 10% of the time, while the three most common isoforms, as a group, occur about 50% of the time. Based on limited information, alternative splicing appears to regulate ligand specificity (13). R&D Systems IL‑17 RC corresponds to IL-17 RC isoform # 3, which shows deletions of aa 36-106 and 264-278 relative to the full-length form (10). Over the ECD, IL‑17 RC isoform #3 is 68% aa identical to mouse IL‑17 RC ECD. IL-17 RC is the cognate receptor for IL-17F, and binds IL-17A with similar affinity (13). With IL‑17 RA, it forms a definitive receptor for both IL-17A and IL-17F. The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL‑17 RA homodimer (4, 9, 13, 15). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
5. You, Z. et al. (2006) Cancer Res. 66:175.
6. You, Z. et al. (2007) Neoplasia 9:464.
7. Gerritsen, M.E. et al. (2003) Br. J. Pharmacol. 140:595.
8. Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
9. Toy, D. et al. (2006) J. Immunol. 177:36.
10. GenBank Accession # Q96F46.
11. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
12. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.
13. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
14. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
15. Kramer, J.M. et al. (2006) J. Immunol. 176:711.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RC Antibody (AF2269)(5)

Publications using AF2269

• B Jin, Y Zhang, HD Miller, L He, D Ge, AR Wang, Z You Defect of IL17 Signaling, but Not Centrinone, Inhibits the Development of Psoriasis and Skin Papilloma in Mouse Models Biomedicines, 2022-08-15;10(8):. 2022-08-15 [PMID: 36009523] (IHC, Human)
• Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Eur J Immunol, 2015-12-14;46(2):440-5. 2015-12-14 [PMID: 26558442] (Western Blot, Human)
• Dongxia Ge, Zongbing You Expression of interleukin-17RC protein in normal human tissues International Archives of Medicine 10/17/2008 [PMID: 18928529]
• Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J. Immunol., 2008-08-15;181(4):2799-805. 2008-08-15 [PMID: 18684971] (Western Blot, Human)
• Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J. Immunol., 2008-01-01;180(1):655-63. 2008-01-01 [PMID: 18097068] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: IL17RC
• Uniprot:
  • NP_116121()