IL-17RB Antibody (152309) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 152309
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

IL-17RB Antibody (152309) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RB
  Arg18-Gly286 (predicted)
  Accession # Q9JIP3
• Specificity: Detects mouse IL‑17 RB in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-17 R, recombinant mouse IL-17 R, or rhIL-17 RB is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RB Antibody (152309) [Alexa Fluor® 488]

• CRL4IL-17 receptor homolog 1
• cytokine receptor CRL4
• Cytokine receptor-like 4
• Evi27
• EVI27IL-17B receptor
• IL-17 RB
• IL-17 receptor B
• IL-17 RH1
• IL17BRinterleukin 17B receptor
• IL-17ER
• IL17RB
• IL-17RB
• IL17Rh1
• IL-17Rh1
• IL17RH1MGC5245
• interleukin 17 receptor B
• interleukin 17 receptor homolog 1
• interleukin-17 receptor B
• Interleukin-17B receptor

Background

The Interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17 RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL-17 RB cDNA encodes a 499 amino acid (aa) type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17 RB also exists (4). Human and mouse IL‑17B R share 76% aa sequence identity. IL-17 RB is aproximately 20% identical to the human and mouse IL‑17 R. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17 RB is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17 RB is significantly upregulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2‑4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.