IL-17F Antibody [Unconjugated]

IL-17F was detected in immersion fixed mouse T helper 17 (Th17) cells stimulated with PMA and calcium ionomycin using Goat Anti-Mouse IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17F Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-17F
  Arg29-Ala153
  Accession # NP_665855
• Specificity: Detects mouse IL-17F in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human
  IL‑17F, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17F
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse IL-17F Antibody (Catalog # AF2057)
  ELISA Detection: Mouse IL-17F Biotinylated Antibody (Catalog # BAF2057)
  Standard: Recombinant Mouse IL-17F (Catalog # 2057-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17F Antibody [Unconjugated]

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

The Interleukin 17 (IL-17) family proteins, comprised of six members (IL-17 and IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17F cDNA encodes a 153 amino acid (aa) protein with a putative 20 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17 sharing approximately 46% aa sequence identity. Mouse and human IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4⁺ T cells and activated monocytes. Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1, 2, 5). The functions for
IL‑17 RC, D, and E are not known. Purified IL-17 R and IL-17B R do not bind IL-17F with high-affinity in vitro. However, binding of IL-17F is detected in cells transfected with IL-17 R, raising the possibility that a co-receptor may be required for IL-17F signaling through IL-17 R (4). The biological activities mediated by IL-17F are similar to those of IL-17. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta , and monocyte chemoattractant protein-1 in endothelial cells (3).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17F Antibody (AF2057)(6)

Publications using AF2057

• P Dhar, GZ Ng, EM Dunne, P Sutton Mucin 1 protects against severe Streptococcus pneumoniae infection Virulence, 2017-07-13;0(0):0. 2017-07-13 [PMID: 28605238] (ELISA Development (Capture), Mouse)
• Shanshan Cai, Sanjay Batra, Ingeborg Langohr, Yochiro Iwakura, Samithamby Jeyaseelan IFN-gamma induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense Mucosal Immunology 5/1/2016 [PMID: 26349661]
• Nam D, Mau E, Wang Y, Wright D, Silkstone D, Whetstone H, Whyne C, Alman B T-Lymphocytes Enable Osteoblast Maturation via IL-17F during the Early Phase of Fracture Repair. PLoS ONE, 2012-06-29;7(6):e40044. 2012-06-29 [PMID: 22768215] (IHC-P, Mouse)
• Meena S. Madhur, Samuel A. Funt, Li Li, Antony Vinh, Wei Chen, Heinrich E. Lob et al. Role of Interleukin 17 in Inflammation, Atherosclerosis, and Vascular Function in Apolipoprotein E–Deficient Mice Arteriosclerosis, Thrombosis, and Vascular Biology 7/1/2011 [PMID: 21474820]
• Meena S. Madhur, Samuel A. Funt, Li Li, Antony Vinh, Wei Chen, Heinrich E. Lob et al. Role of Interleukin 17 in Inflammation, Atherosclerosis, and Vascular Function in Apolipoprotein E–Deficient Mice Arteriosclerosis, Thrombosis, and Vascular Biology 2011-07-01 [PMID: 21474820] (Neutralization, Transgenic Mouse)
• Ishigame H, Kakuta S, Nagai T, Kadoki M, Nambu A, Komiyama Y, Fujikado N, Tanahashi Y, Akitsu A, Kotaki H, Sudo K, Nakae S, Sasakawa C, Iwakura Y Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses. Immunity, 2009-01-16;30(1):108-19. 2009-01-16 [PMID: 19144317] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL17F
• Uniprot:
  • NP_665855(Mouse)