IL-17F Antibody (775620) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: B/N
• Clone: 775620
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

IL-17F Antibody (775620) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-17F
  Gly21-Thr153
  Accession # Q96PD4
• Specificity: Detects human IL-17F in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-17A, recombinant mouse IL-17F, or recombinant rat IL-17F is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17F Antibody (775620) [Alexa Fluor® 405]

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine‑knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16 - 30%) with IL-17B, C, D and E. Human and mouse IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T-cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL-17A and IL-17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL-17F are similar to those of IL‑17. IL‑17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL‑17F also inhibits angiogenesis and induces production of IL-2, TGF-beta , and monocyte chemoattractant protein-1 in endothelial cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.