Recombinant Mouse IL-17E/IL-25 Animal-Free Protein

SDS-Page: Recombinant Mouse IL-17E/IL-25 Animal-Free Protein [NBP2-76251] - 1 ug run under (-) non-reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. Mouse IL-17E is a homodimer with a predicted MW of 35.2 kDa (each monomer is 17.6 kDa).

Product Discontinued

Recombinant Mouse IL-17E/IL-25 Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: IL-17E/IL-25 is a dimer, 17.6/35.2 kDa (154/308 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL25
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 35.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Sterile 10 mM HCI at 0.1 mg/mL

Alternate Names for Recombinant Mouse IL-17E/IL-25 Animal-Free Protein

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL25