IL-16 Antibody (228109) [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clone: 228109
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-16 Antibody (228109) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-16
  Ser1205-Ser1322
  Accession # O54824
• Specificity: Detects mouse IL-16 in direct ELISAs and Western blots. Shows approximately 50% cross‑reactivity with recombinant human (rh) IL-16 and no cross-reactivity with rmC-10, rhI-309, rhHCC-4, or rhHCC-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL16
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-16 Antibody (228109) [Unconjugated]

• FLJ16806
• FLJ42735
• FLJ44234
• HsT19289
• IL16
• IL-16
• interleukin 16 (lymphocyte chemoattractant factor)
• LCF
• LCFprIL-16
• lymphocyte chemoattractant factor
• neuronal interleukin 16
• NIL16
• PRIL16
• prointerleukin 16
• pro-interleukin-16

Background

Interleukin 16 (IL-16), also known as lymphocyte chemoattractant factor (LCF), refers to the C-terminal peptide of pro-IL-16 isoform 1, which is a nuclear and cytoplasmic PDZ-containing protein. The C-terminus of pro-IL-16 is cleaved by Caspase-3, resulting in the release of secreted IL-16. Mature IL-16 has been shown to bind the cell surface receptor CD4 and functions as an immunomodulatory cytokine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-16 Antibody (MAB1727)(2)

Publications using MAB1727

• Y Chang, YM Hsiao, CC Hu, CH Chang, CY Li, SWN Ueng, MF Chen Synovial Fluid Interleukin-16 Contributes to Osteoclast Activation and Bone Loss through the JNK/NFATc1 Signaling Cascade in Patients with Periprosthetic Joint Infection Int J Mol Sci, 2020-04-21;21(8):. 4/21/2020 [PMID: 32326301]
• Shunsuke Tamaki, Toshiaki Mano, Yasushi Sakata, Tomohito Ohtani, Yasuharu Takeda, Daisuke Kamimura et al. Interleukin-16 Promotes Cardiac Fibrosis and Myocardial Stiffening in Heart Failure with Preserved Ejection Fraction PLoS ONE 2013-07-19 [PMID: 23894370]

Bioinformatics

• Gene Symbol: IL16
• Uniprot:
  • O54824()