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AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
HAF017). Specific bands were detected for IL-16 at approximately 14 kDa (active) and 45-55 kDa (precursor), as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
Species: Hu Applications: CyTOF-ready, Flow Host: Mouse Monoclonal
Interleukin-16 (IL-16, also known as lymphocyte chemoattractant factor (LCF)) is a CD8+ T cell-derived cytokine. It acts as a chemoattractant for CD4+ T-cells, monocytes and eosinophils. IL-16 has also been shown to suppress HIV-1 replication in vitro. It is expressed as a precursor molecule (pro-IL-16) in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The active form is assumed to be a proteolytic cleavage product of pro-IL-16 existing, under physiological conditions, predominantly as a noncovalently linked multimer. The sequence and structure of IL-16 is conserved across species, human and murine IL-16 show significant cross-species reactivity.
![Flow Cytometry IL-16 Antibody [Unconjugated] - C-terminal Peptide](http://images.novusbio.com/images/antibody/af-316-pb_human-il-16-affinity-purified-polyclonal-ab-flow-cytometry-306202593929..jpg)
![Flow Cytometry IL-16 Antibody (70720) [Unconjugated]](http://images.novusbio.com/images/antibody/mab3161_human-il-16-mab-clone-70720-flow-cytometry-306202593537..jpg)
![ELISA IL-16 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-16-Antibody-Pair-[HRP]-ELISA-NBP3-11656-img0001.jpg)
![ELISA IL-16 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-16-Antibody-Pair-[HRP]-ELISA-NBP3-11655-img0001.jpg)
![N/A IL-16 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL16_D1600_ELISA_537.jpg)
![N/A IL-16 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL16_DY1727_ELISA_1704.jpg)
![N/A IL-16 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL16_DY316_ELISA_1921.jpg)
![Western Blot IL-16 Antibody [Unconjugated] - C-terminal Peptide](http://images.novusbio.com/images/antibody/IL16_AF316PB_Western_Blot_20238.jpg)
![Immunocytochemistry IL-16 Antibody [Unconjugated] - C-terminal Peptide](http://images.novusbio.com/images/antibody/IL-16_AF-316-PB_Immunocytochemistry_6464.jpg)
![Immunohistochemistry IL-16 Antibody [Unconjugated] - C-terminal Peptide](http://images.novusbio.com/images/antibody/IL-16_AF-316-PB_Immunohistochemistry_9926.jpg)
![N/A IL-16 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL16_D1600_ELISA_538.jpg)
![N/A IL-16 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-16_D1600_ELISA_14.jpg)
