Mouse IL-13 ELISA Kit (Colorimetric)

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ELISA: IL-13 ELISA Kit [Biotin] [NBP1-92663] - Standard curve

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-92663
    • Availability
      Product Discontinued

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Mouse IL-13 ELISA Kit (Colorimetric) Summary

Description
The mouse IL-13 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-13
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with the inactive IL-13/IL-13BP complex.
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
2.8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 5.00%
Intra-Assay
CV% < 4.60%
Spike Recovery
The overall mean recovery of about 10% might be due to IL-13/IL13BP complex formation. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL13

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 bottle (10 mL) Assay Buffer A Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  2. 1 bottle (25 mL) Sample Diluent Z
  3. 1 bottle (25 mL) Tetramethylbenzidine (TMB) Substrate Solution
  4. 1 vial (100 uL) Capture Antibody Concentrate (250 x)
  5. 1 vial (2.5 mL) Phosphate Buffered Saline Concentrate (PBS) 10x
  6. 1 vial (50 uL) Detection Antibody Concentrate (250 x)
  7. 2 96-well plates
  8. 2 vials mouse IL-19 standard, (lyophilized), 8000 pg/mL upon reconstitution

Alternate Names for Mouse IL-13 ELISA Kit (Colorimetric)

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

Interleukin-13 is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. IL-13 is an immunoregulatory cytokine and a key mediator in the pathogenesis of allergic inflammation. It mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation. The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IL-13 ELISA Kit (NBP1-92663). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

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Bioinformatics

Gene Symbol IL13