Mouse IL-13 ELISA Kit (Colorimetric)

ELISA: IL-13 ELISA Kit [Biotin] [NBP1-92663] - Standard curve

Product Discontinued

Mouse IL-13 ELISA Kit (Colorimetric) Summary

• Description: The mouse IL-13 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-13
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with the inactive IL-13/IL-13BP complex.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 2.8 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.00%
• Intra-Assay: CV% < 4.60%
• Spike Recovery: The overall mean recovery of about 10% might be due to IL-13/IL13BP complex formation. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL13

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-13 ELISA Kit (Colorimetric)

• ALRHMGC116789
• BHR1interleukin-13
• IL13
• IL-13
• IL-13MGC116788
• interleukin 13
• MGC116786
• NC30
• P600

Background

Interleukin-13 is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. IL-13 is an immunoregulatory cytokine and a key mediator in the pathogenesis of allergic inflammation. It mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation. The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-13 ELISA Kit (NBP1-92663). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL13