Mouse IL-13 ELISA Kit (Colorimetric)

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ELISA: IL-13 ELISA Kit [Biotin] [NBP1-92663] - Standard curve

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate)
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
2.8 pg/mL

Order Details

Mouse IL-13 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with the inactive IL-13/IL-13BP complex.
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
2.8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.00%
Intra-Assay
4.60%
Spike Recovery
The overall mean recovery of about 10% might be due to IL-13/IL13BP complex formation. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL13

Applications/Dilutions

Application Notes
The mouse IL-13 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-13

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 vial (50 uL) Detection Antibody Concentrate (250 x)
  2. 1 vial (2.5 mL) Phosphate Buffered Saline Concentrate (PBS) 10x
  3. 1 vial (100 uL) Capture Antibody Concentrate (250 x)
  4. 1 bottle (10 mL) Assay Buffer A Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  5. 1 bottle (25 mL) Sample Diluent Z
  6. 1 bottle (25 mL) Tetramethylbenzidine (TMB) Substrate Solution
  7. 2 vials mouse IL-19 standard, (lyophilized), 8000 pg/mL upon reconstitution
  8. 2 96-well plates

Alternate Names for Mouse IL-13 ELISA Kit (Colorimetric)

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

Interleukin-13 is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. IL-13 is an immunoregulatory cytokine and a key mediator in the pathogenesis of allergic inflammation. It mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation. The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL13
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