Human IL-13 ELISA Kit (Colorimetric)

Human IL-13 ELISA Kit [NBP1-91176] - Data for Human IL-13 Platinum ELISA

Product Discontinued

Human IL-13 ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit has been transitioned to a new version, available here: D1300B
• Specificity: The assay detects both natural and recombinant human IL-13. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-13 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 0.7 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.60%
• Intra-Assay: CV% < 6.00%
• Spike Recovery: The recovery ranged from 93% to 110% with an overall mean recovery of 101%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL13

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-13 ELISA Kit (Colorimetric)

• ALRHMGC116789
• BHR1interleukin-13
• IL13
• IL-13
• IL-13MGC116788
• interleukin 13
• MGC116786
• NC30
• P600

Background

Human IL-13 is a nonglycosylated protein of 132 amino acids with a molecular weight of 12 kDa. The IL-13 gene is located on chromosome 5q23-31, in the same region as the genes encoding IL-3, IL-4, IL-5 and GM-CSF. It is produced by activated CD4+ and CD8+ T cells, but the expression seems to be more abundant in CD4+ T cells. Although it is a Th2 cytokine in the mouse, it appears to be produced by Th0, Th1, and Th2-like human T cell clones. IL-13 induces CD23 expression on B cells, promotes B cell proliferation in combination with anti-Ig or CD40 antibodies, and stimulates secretion IgE, and IgG4. IL-13 has also been shown to prolong survival of human monocytes and increases surface expression of MHC class II, CD23, and members of the integrin superfamily, like CD11b, CD11c, CD18, CD29 and CD49e. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. The capacity of IL-13 to induce IgE synthesis indicates that IL-13 may play an important regulatory role in IgE-mediated atopic diseases. The measurement of IL-13 in body fluids may thus provide further information about the pathophysiology of atopic diseases. Furthermore, IL-13 inhibits HIV-1 replication in primary culture-derived macrophages and represents a candidate cytokine for the suppression of HIV infection within monocytes and macrophages in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-13 ELISA Kit (NBP1-91176). (Showing 1 - 2 of 2 FAQ).

1. Does this kit allow for detection of IL-13 bound to a soluble receptor?
   • Our human IL-13 ELISA kit with catalogue number NBP1-91176 is intended for the quantitative detection of human IL-13, and suitable sample types include cell culture supernatant, serum, plasma and amniotic fluid. An anti-human IL-13 coating antibody is adsorbed onto microwells, and any human IL-13 present in the sample binds to this antibody; biotin-conjugated anti-human IL-13 antibody and streptavidin-HRP are then used for detection. A purified human IL-13 standard (provided with the kit) is used as a control. Unfortunately we have no specific testing data to confirm whether the antibodies in this kit would be able to detect IL-13 once it has become bound to a soluble receptor. Since we have not epitope-mapped the antibodies that are used in the kit, we do not know exactly where on the IL-13 protein they bind, and if the receptor was to block these binding sites then the kit would not work optimally.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL13