IL-13 Antibody (31606) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
31606
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

IL-13 Antibody (31606) [Alexa Fluor® 405] Summary

Immunogen
E. coli-derived recombinant human IL-13
Gly35-Asn146
Accession # P35225
Specificity
Detects human IL-13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL‑4 or recombinant mouse IL-13 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL13
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13 Antibody (31606) [Alexa Fluor® 405]

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL13
Entrez
Uniprot