IL-12 R beta 2 Antibody (305719) [Allophycocyanin]

Human CD4+peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100), 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL), and 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human/Mouse IL-12 R beta 2 APC-conjugated Monoclonal Antibody (Catalog # FAB1959A). Quadrant markers were set based on control antibody staining using Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 305719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

IL-12 R beta 2 Antibody (305719) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
  Cys28-Asn622
  Accession # Q99665
• Specificity: Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12RB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 2 Antibody (305719) [Allophycocyanin]

• IL-12 R beta 2
• IL-12 receptor beta 2
• IL-12 receptor subunit beta-2
• IL12R beta 2
• IL-12R subunit beta-2
• IL12RB2
• IL-12Rb2
• IL-12R-beta-2
• interleukin 12 receptor, beta 2
• interleukin-12 receptor beta-2 chain
• interleukin-12 receptor subunit beta-2

Background

Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma . It is also required for the expansion and activation Th1 cells (1, 2).

The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).

1. Trinchieri, G. et al. (2003) Immunity 19:641. 
2. Brombacher, F. et al. (2003) Trends in Immunol. 23:207. 
3. Trinchieri, G. (2003) Nature Reviews Immunol. 3:133.
4. Rogge, L. et al. (1997) J. Exp. Med. 185:825.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 R beta 2 Antibody (FAB1959A)(7)

Publications using FAB1959A

• Surya P. Pandey, Mackenzie J. Bender, Alex C. McPherson, Catherine M. Phelps, Luzmariel Medina Sanchez, Mohit Rana et al. Tet2 deficiency drives liver microbiome dysbiosis triggering Tc1 cell autoimmune hepatitis Cell Host & Microbe 7/1/2022 [PMID: 35658976]
• Amardeep Singh, Yousif Subhi, Marie Krogh Nielsen, Mads Krüger Falk, Sara Maj Hyldig Matzen, Finn Sellebjerg et al. Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration: A case-control study Scientific Reports 4/4/2017 [PMID: 28377586]
• Lars Fugger Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity Sci Transl Med, 2016-11-02;8(363):363ra149. 2016-11-02 [PMID: 27807284] (Flow Cytometry, Human)
• Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue Acta Neuropathol Commun, 2016-09-06;4(1):100. 2016-09-06 [PMID: 27600707] (Flow Cytometry, Mouse)
• Mamata S Gokhale, Vladimir Vainstein, Jamie Tom, Simmy Thomas, Chris E Lawrence, Zoya Gluzman-Poltorak et al. Single low-dose rHuIL-12 safely triggers multilineage hematopoietic and immune-mediated effects Experimental Hematology & Oncology 12/1/2014 [PMID: 24725395]
• Liu Y, Xu Y, Sun J, Ma A, Zhang F, Xia S, Xu G, Liu Y AKT hyperactivation confers a Th1 phenotype in thymic Treg cells deficient in TGF-beta receptor II signaling. Eur J Immunol, 2013-12-02;44(2):521-32. 2013-12-02 [PMID: 24165986] (Flow Cytometry, Mouse)
• Liao W, Lin JX, Wang L, Li P, Leonard WJ Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages. Nat. Immunol., 2011-04-24;12(6):551-9. 2011-04-24 [PMID: 21516110] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL12RB2
• Uniprot:
  • Q99665()