IL-12 R beta 1 Antibody [Biotin]

Murine p40 with D36K/W37K/T38K substitutions interacts with murine p19 but not with murine IL-12R beta 1.A, structure of hIL-23 extracted from the structure of the IL-23:IL-23R complex structure (PDB 5mzv). A flexible linker sequence connecting p40 and p19 is indicated. B, Western blot analysis of secreted murine HIL-23, mp19 and mp40 variants from transfected CHO-K1 cells. The position of mp19 and mp40 variants is indicated by arrows. C, cellular proliferation of Ba/F3-gp130-mIL-12R beta 1-mIL-23R cells. The cells were cultured for 3 days in the presence of 10 ng/ml HIL-6 or with the indicated cytokines (10% conditioned cell culture supernatant of transfected CHO-K1 cells). Parental Ba/F3-gp130 cells were used as controls. The results of one representative experiment of three are shown. Error bars represent S.D. for technical replicates. Statistical analysis used a one-way ANOVA, followed by Bonferroni correction (n = 3), ∗∗∗p ≤ 0.001. D, analysis of STAT3 and ERK1/2 activation. Ba/F3-gp130-mIL-12R beta 1-mIL-23R cells were washed, starved, and stimulated with the indicated cytokines (10% conditioned cell culture supernatant of transfected CHO-K1 cells) for 30 min. Cellular lysates were prepared, and equal amounts of total protein (50 μg/lane) were loaded on SDS-PAA gels, followed by immunoblotting using specific antibodies for phospho-STAT3, STAT3, phospho-ERK1/2, and ERK1/2. Western blotting data show results of one representative experiment of two. E, co-IP of FLAG-tagged murine mp19 coexpressed with either mp40 or mp40D36K/W37K/T38K and full-length mIL-12R beta 1 or mIL-23R. The position of mIL-23R and mIL-12R beta 1 is indicated by arrows. One of two independent experiments is shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34637790), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-12 R beta 1 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
  Val32-Glu561
  Accession # Q60837
• Specificity: Detects mouse IL-12 R beta 1 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IL-12 R beta 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL12RB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 1 Antibody [Biotin]

• CD212 antigen
• CD212
• IL-12 R beta 1
• IL-12 receptor beta component
• IL-12 receptor subunit beta-1
• IL12R beta 1
• IL-12R subunit beta-1
• IL12R
• IL12RB
• IL12RB1
• IL-12Rb1
• IL-12R-BETA1
• IL-12R-beta-1
• interleukin 12 receptor, beta 1
• interleukin-12 receptor beta-1 chain
• interleukin-12 receptor subunit beta-1
• MGC34454

Background

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL-12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%-21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4-6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8-10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL-23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.
4. Parham, C. et al. (2002) J. Immunol. 168:5699.
5. Wu, C. et al. (1997) J. Immunol. 159:1658.
6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.
10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.
11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
13. Shimozato, O. et al. (2006) Immunology 117:22.
14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 R beta 1 Antibody (BAF1998)(2)

Publications using BAF1998

• J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction The Journal of Biological Chemistry, 2021-10-09;0(0):101295. 2021-10-09 [PMID: 34637790] (Western Blot, Mouse)
• Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl et al. Defining the functional binding sites of interleukin 12 receptor beta 1 and interleukin 23 receptor to Janus kinases Molecular Biology of the Cell 7/15/2016 [PMID: 27193299]

Bioinformatics

• Gene Symbol: IL12RB1
• Uniprot:
  • Q60837()