IL-12 R beta 1 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-12 R beta 1 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
  Val32-Glu561
  Accession # Q60837
• Specificity: Detects mouse IL-12 R beta 1 in direct ELISAs and Western blots. In these formats, approximately 20% cross-reactivity with recombinant human IL-12 R beta 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL12RB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 1 Antibody [Unconjugated]

• CD212 antigen
• CD212
• IL-12 R beta 1
• IL-12 receptor beta component
• IL-12 receptor subunit beta-1
• IL12R beta 1
• IL-12R subunit beta-1
• IL12R
• IL12RB
• IL12RB1
• IL-12Rb1
• IL-12R-BETA1
• IL-12R-beta-1
• interleukin 12 receptor, beta 1
• interleukin-12 receptor beta-1 chain
• interleukin-12 receptor subunit beta-1
• MGC34454

Background

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL-12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16% - 21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4 - 6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8 - 10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL-23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.
4. Parham, C. et al. (2002) J. Immunol. 168:5699.
5. Wu, C. et al. (1997) J. Immunol. 159:1658.
6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.
10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.
11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
13. Shimozato, O. et al. (2006) Immunology 117:22.
14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 R beta 1 Antibody (AF1998)(1)

Publication using AF1998

• Ray A, Fountain J, Miller H, Cooper A, Robinson R IL12Rbeta1DeltaTM is a secreted product of il12rb1 that promotes control of extrapulmonary tuberculosis. Infect Immun, 2014-11-17;83(2):560-71. 2014-11-17 [PMID: 25404030] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL12RB1
• Uniprot:
  • Q60837()