IL-12 R beta 1 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-12 R beta 1 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
Val32-Glu561
Accession # Q60837
Specificity
Detects mouse IL-12 R beta 1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant human IL-12 R beta 1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL12RB1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF1998 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 1 Antibody

  • CD212 antigen
  • CD212
  • IL-12 R beta 1
  • IL-12 receptor beta component
  • IL-12 receptor subunit beta-1
  • IL12R beta 1
  • IL-12R subunit beta-1
  • IL12R
  • IL12RB
  • IL12RB1
  • IL-12Rb1
  • IL-12R-BETA1
  • IL-12R-beta-1
  • interleukin 12 receptor, beta 1
  • interleukin-12 receptor beta-1 chain
  • interleukin-12 receptor subunit beta-1
  • MGC34454

Background

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16% - 21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4 - 6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8 - 10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

  1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
  2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.
  4. Parham, C. et al. (2002) J. Immunol. 168:5699.
  5. Wu, C. et al. (1997) J. Immunol. 159:1658.
  6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
  7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
  8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
  9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.
  10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.
  11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
  12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
  13. Shimozato, O. et al. (2006) Immunology 117:22.
  14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1998
Species: Mu
Applications: WB

Publications for IL-12 R beta 1 Antibody (AF1998)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1998
Phycoerythrin FAB1998P
Phycoerythrin FAB1998P

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Bioinformatics

Gene Symbol IL12RB1
Uniprot