IL-12/IL-23 p40 Antibody (31052) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 31052
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-12/IL-23 p40 Antibody (31052) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12/IL-23 p40 free monomer
  Ile23-Ser328
  Accession # P29460
• Specificity: Detects human IL-12/IL-23 p40 in ELISAs. In ELISAs, this antibody shows less than 5% cross-reactivity with recombinant human (rh) IL‑12 (p35/p40 heterodimer) and rhIL-23 (p19/p40 heterodimer). No significant cross-reactivity or interference was observed with rhIL-1 alpha , rmIL-1 alpha , rhIL-1 beta , rmIL-1 beta , rhIL-1ra, rhIL-1 RI, rhIL-1 RII, rhIL-2, rhIL-2 R alpha , rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-4 R, rhIL-5, rmIL-5, rhIL-5 R beta , rhIL-6, rhIL-6 R, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, rmIL-10, rhIL-11, rmIL-12, rmIL-12 p40, rhIL-13, or rmIL-13.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12B
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human IL-12 Biotinylated Antibody (Catalog number BAF219)
  Standard: Recombinant Human IL-12/IL-23 p40 Monomer (Catalog number 309-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (31052) [Unconjugated]

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a
disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL‑12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-23 p40 Antibody (MAB609)(2)

Publications using MAB609

• Jindra C Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Eur J Immunol, 2010-02-01;40(2):321-9. 2010-02-01 [PMID: 19950173] (Immunoprecipitation, Human)
• Duraisingham SS, Hornig J, Gotch F, Patterson S TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Th17 polarization of naive T cells but do stimulate Th1 and Th17 memory responses. J. Immunol., 2009-07-22;183(4):2242-51. 2009-07-22 [PMID: 19625644] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL12B
• Uniprot:
  • P29460()