IL-12/IL-23 p40 Antibody (30517) [Unconjugated]

• Reactivity: Mu
• Applications: ELISA(Cap)
• Clone: 30517
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-12/IL-23 p40 Antibody (30517) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12 p40
• Specificity: Detects mouse IL-12 p40 in ELISAs. Polymorphisms exist in the mouse IL-12 p40 sequence. This antibody recognizes an allelic epitope found in the p40 subunit of several mouse strains including BALB/c, C57BL/6, CBA, C3H, DBA/1, NOR, I29/J, and A.SW. This antibody does not recognize the allelic variant that occurs in strains AKR, DBA/2, NOD, NZB, NZC, NZO, NZW, or SJL (1). In ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) IL-23, 30% cross-reactivity with rmIL-12 p70, and no cross-reactivity with recombinant human (rh) IL-12 p70 or rhIL-12 p40.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Il12b
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Mouse IL-12/IL-23 p40 Biotinylated Antibody (Catalog number BAF499)
  Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog number 499-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (30517) [Unconjugated]

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

1. Ymer, S. et al. (2002) Genes and Immunity 3:151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-23 p40 Antibody (MAB499)(3)

Publications using MAB499

• Schroder J, Moll J, Baran P, Grotzinger J, Scheller J, Floss D Non-canonical interleukin 23 receptor complex assembly: p40 protein recruits interleukin 12 receptor beta1 via site II and induces p19/interleukin 23 receptor interaction via site III. J Biol Chem, 2014-11-04;290(1):359-70. 2014-11-04 [PMID: 25371211] (Immunoprecipitation, Mouse, Primate - Chlorocebus aethiops (African Green Monkey))
• Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice. J. Immunol., 2007-12-15;179(12):8274-9. 2007-12-15 [PMID: 18056371] (Neutralization, Mouse)
• Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol., 2002-04-01;160(4):1353-60. 2002-04-01 [PMID: 11943720] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Il12b