IL-12/IL-23 p40 Antibody (24901)

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In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL‑23 (Catalog # 1290-IL) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N
Clone
24901
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-12/IL-23 p40 Antibody (24901) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p40/p35 heterodimer
Specificity
Detects human IL-12 (p40/p35 heterodimer), IL-23 (p40/p19 heterodimer), and p40 homodimers in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-12 p40 or rpIL-12.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12B
Purity
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.02-0.1 ug/mL
Publications
Read Publications using
MAB1510 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (24901)

  • CLMF p40
  • CLMF
  • CLMF2
  • Cytotoxic lymphocyte maturation factor 40 kDa subunit
  • IL12 p40
  • IL-12 p40
  • IL-12 subunit p40
  • IL12B
  • IL-12B
  • IL-12BNK cell stimulatory factor chain 2
  • interleukin 12, p40
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
  • interleukin-12 beta chain
  • interleukin-12 subunit beta
  • natural killer cell stimulatory factor, 40 kD subunit
  • NKSF
  • NKSF2
  • NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: B/N

Publications for IL-12/IL-23 p40 Antibody (MAB1510)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Functional Assay, Neutralization.


Filter By Application
Functional Assay
(1)
Neutralization
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB1510 Applications Species
MH Abumaree, E Bahattab, A Alsadoun, A Al Dosaima, FM Abomaray, T Khatlani, B Kalionis, MF El-Muzaini, AO Alawad, AS AlAskar Characterization of the interaction between human decidua parietalis mesenchymal stem/stromal cells and natural killer cells Stem Cell Res Ther, 2018;9(1):102. 2018 [PMID: 29650045] (Neutralization, Human) Neutralization Human
MS Packer, HA Rees, DR Liu Phage-assisted continuous evolution of proteases with altered substrate specificity Nat Commun, 2017;8(1):956. 2017 [PMID: 29038472] (Neutralization, Mouse) Neutralization Mouse
Sommer A, Fabri M Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE, 2015;10(6):e0130395. 2015 [PMID: 26107738] (Neutralization, Human) Neutralization Human
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects. J Immunol Res, 2014;2014(0):593562. 2014 [PMID: 24741609] (Functional Assay, Human) Functional Assay Human
Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. J Immunol, 2012;189(12):5590-601. 2012 [PMID: 23152566] (Neutralization, Human) Neutralization Human
Rao DA, Tracey KJ, Pober JS IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. J. Immunol., 2007;179(10):6536-46. 2007 [PMID: 17982042] (Neutralization, Human) Neutralization Human
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism. J. Immunol., 2005;174(4):2376-86. 2005 [PMID: 15699174] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol IL12B