IL-12/IL-23 p40 Antibody (169516) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 169516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-12/IL-23 p40 Antibody (169516) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑23 p19/p40 heterodimer
  Ile23-Ser328
  Accession # P29460
• Specificity: Detects human IL-12/IL-23 p40 subunit in direct ELISAs and Western blots. Recognizes IL-12 and IL-23 heterodimers as well as p40 homodimers. Shows 100% cross-reactivity with porcine IL-12/IL-23 p40 and no cross-reactivity with mouse IL-12/IL-23 p40.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (169516) [Unconjugated]

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-23 p40 Antibody (MAB6091)(6)

Publications using MAB6091

• P Pandya, RO Sayers, JP Ting, S Morshedian, C Torres, JS Cudal, K Zhang, JR Fitchett, Q Zhang, FF Zhang, J Wang, JD Durbin, JJ Carrillo, A Espada, H Broughton, Y Qian, S Afshar Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage PLoS ONE, 2020-06-01;15(6):e0233961. 2020-06-01 [PMID: 32479512] (Flow Cytometry, Yeast)
• KE van Meijga, B Khatri, SG Smith, AMFH Drittij, RA de Paus, JJ Goeman, MM Ho, HM Dockrell, H McShane, SA Joosten, THM Ottenhoff Cross-laboratory evaluation of multiplex bead assays including independent common reference standards for immunological monitoring of observational and interventional human studies PLoS ONE, 2018-09-04;13(9):e0201205. 9/4/2018 [PMID: 30180167]
• KOJI FUKUDA, YASUSHI MIURA, TOSHIHISA MAEDA, SHINYA HAYASHI, MASAHIRO KUROSAKA Interleukin-12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts Molecular Medicine Reports 4/1/2016 [PMID: 26956410]
• KOJI FUKUDA, YASUSHI MIURA, TOSHIHISA MAEDA, SHINYA HAYASHI, MASAHIRO KUROSAKA Interleukin-12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts Molecular Medicine Reports 2016-04-01 [PMID: 26956410] (Western Blot, Human)
• Yu RY, Gallagher G A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells. J. Immunol., 2010-11-12;185(12):7302-8. 2010-11-12 [PMID: 21076058] (Western Blot, Human)
• Vaknin-Dembinsky A, Balashov K, Weiner HL IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production. J. Immunol., 2006-06-15;176(12):7768-74. 2006-06-15 [PMID: 16751425] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL12B
• Uniprot:
  • P29460()