IL-12/IL-23 p40 Antibody (116224) [Unconjugated]

• Reactivity: Po
• Applications: ELISA(Cap)
• Clone: 116224
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-12/IL-23 p40 Antibody (116224) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer
• Specificity: Detects the p40 subunit of porcine IL-12/IL-23 in ELISAs and Western blots. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human IL‑12 and IL-23 and no cross-reactivity with recombinant mouse IL-12 or IL-23.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Porcine IL-12/IL-23 p40 Biotinylated Antibody (Catalog number BAM9122)
  Standard: Recombinant Porcine IL-12 (Catalog number 912-PL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (116224) [Unconjugated]

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-23 p40 Antibody (MAB9121)(1)

Publication using MAB9121

• S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA Development (Capture), Porcine)