Mouse IL-11 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1792
    • Availability
      Product Discontinued

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Mouse IL-11 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. Il11 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Il11.
Sensitivity
1 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL11

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.
Publications
Read Publication using KA1792.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent B (Item E): 5x concentrated buffer. For detection antibodyand HRP-Streptavidin diluent.
  2. Assay Diluent D (Item K): 5x concentrated buffer. For Standard/Sample(serum/plasma samples/cell culture medium) diluent.
  3. Detection Antibody IL-11 (Item F): Biotinylated anti- mouse IL-11 (each vialis enough to assay half microplate).
  4. HRP-Streptavidin concentrate (Item G): 400x concentratedHRP-conjugated Streptavidin.
  5. IL-11 Microplate (Item A): Coated with anti-mouse IL-11
  6. Standards: Recombinant mouse IL-11 (Item C)
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse IL-11 ELISA Kit (Colorimetric)

  • Adipogenesis inhibitory factor
  • AGIF
  • AGIFoprelvekin
  • IL11
  • IL-11
  • IL-11Oprelvekin
  • interleukin 11
  • interleukin-11
  • Oprelvekin

Background

Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production.Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for IL-11 ELISA Kit (KA1792)(1)

We have publications tested in 1 confirmed species: Mouse.


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Product General Protocols

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FAQs for IL-11 ELISA Kit (KA1792). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Additional IL-11 Products

Research Areas for IL-11 ELISA Kit (KA1792)

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Bioinformatics

Gene Symbol IL11