IL-11 Antibody (22626R) [Unconjugated]

Recombinant Human IL-11 (218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-11 Monoclonal Antibody (Catalog # MAB218R). The ND50 is typically 5-15 µg/mL

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 22626R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-11 Antibody (22626R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human IL‑11
  Pro22-Leu199
  Accession # P20809
• Specificity: Detects human IL-11 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-11 Antibody (22626R) [Unconjugated]

• Adipogenesis inhibitory factor
• AGIF
• AGIFoprelvekin
• IL11
• IL-11
• IL-11Oprelvekin
• interleukin 11
• interleukin-11
• Oprelvekin

Background

Interleukin-11 (IL-11) is a pleiotropic cytokine produced by stromal cells. It binds to a dimeric receptor complex consisting of the IL-11 Ra chain and the gp130 chain shared by the receptors for the IL-6 cytokine family. IL-11 has multiple effects on hematopoietic cells and osteoblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P20809()