Recombinant Mouse IL-1 beta/IL-1F2 Protein

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SDS-Page: Mouse IL-1 beta/IL-1F2 Protein [NBP2-35109]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Mouse IL-1 beta/IL-1F2 Protein Summary

Description
A biologically active protein to IL1B.

Source: E. coli

Amino Acid Sequence: VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS
Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
IL1 beta Protein is Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 x 10^8 IU/mg
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL1B
Purity
>96%, by HPLC
Endotoxin Note
Less than 1EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-35109.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
>96%, by HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

Interleukin-1beta (IL-1beta) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1alpha and IL-1beta binds to the same receptor and has similar but not identical biological properties. The mature mouse IL1beta shares 90 % amino acid sequence identity with cotton rat and rat and 65 %-78 % identity with canine, human and rhesus IL1beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for IL-1 beta/IL-1F2 Recombinant Protein (NBP2-35109)(1)

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Uniprot