IL-1 beta/IL-1F2 Antibody [Unconjugated]

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201‑LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Human IL‑1 beta /IL‑1F2 Poly­clonal Antibody (Catalog #AB‑201‑NA). The ND50 is typically 0.05‑0.1 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-1 beta /IL-1F2
  Ala117-Ser269
  Accession # NP_000567
• Specificity: Detects human IL-1 beta /IL-1F2 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant rhesus monkey is observed, and approximately 50% cross-reactivity with recombinant mouse IL-1 beta , recombinant rat IL-1 beta , recombinant canine IL-1 beta , recombinant equine IL-1 beta , recombinant feline IL-1 beta , and approximately 20% cross-reactivity with recombinant porcine IL-1 beta and recombinant cotton rat IL-1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.05-0.1 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is covered by one or more of the following U.S. patents: 4,766,069, 5,510,462, 5,681,933, 4,762,914, 5,474,899, 5,789,185, 5,484,887, 5,122,459, 5,001,057, 5,077,219, 5,286,847. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (AB-201-NA)(7)

Publications using AB-201-NA

• NG Díaz-Marot, G Garcia-Vic, G Polcaro, M Bañuls, N Albert, A Villanueva, DG Molleví The Blockade of Tumoral IL1&amp;beta-Mediated Signaling in Normal Colonic Fibroblasts Sensitizes Tumor Cells to Chemotherapy and Prevents Inflammatory CAF Activation International Journal of Molecular Sciences, 2021-05-07;22(9):. 2021-05-07 [PMID: 34066976] (Neutralization, Human)
• Hirokazu Amamizu, Yasuharu Matsumoto, Susumu Morosawa, Kazuma Ohyama, Hironori Uzuka, Michinori Hirano et al. Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology 4/1/2019 [PMID: 30816801]
• Masafumi Okuda, Jun Inoue, Naoto Fujiwara, Tatsuyuki Kawano, Johji Inazawa Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection Oncotarget 5/23/2017 [PMID: 28410227]
• Masafumi Okuda, Jun Inoue, Naoto Fujiwara, Tatsuyuki Kawano, Johji Inazawa Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection Oncotarget 2017-05-23 [PMID: 28410227] (Western Blot, Human)
• Mingfang Zhang, Yuanlin Qi, Hui Li, Jiajun Cui, Lu Dai, Jacqueline A. Frank et al. AIM2 inflammasome mediates Arsenic-induced secretion of IL-1 beta and IL-18 OncoImmunology 6/2/2016 [PMID: 27471628]
• Thomas Lettner, Roland Lang, Alfred Klausegger, Stefan Hainzl, Johann W. Bauer, Verena Wally MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex PLoS ONE 7/19/2013 [PMID: 23894602]
• Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells. Blood, 2000-06-15;95(12):3758-64. 2000-06-15 [PMID: 10845907] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • NP_000567()