IL-1 beta/IL-1F2 Antibody (Hu110) [Unconjugated]

Human peripheral blood monocytes treated with 500 ng/mL LPS overnight, were either (A) stained or (B) not stained with Human anti-Human IL-1 beta/IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody (Catalog # MAB10349) followed by APC-conjugated anti-Human IgG secondary antibody (F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: Hu110
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

IL-1 beta/IL-1F2 Antibody (Hu110) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: Human IL‑1 beta /IL‑1F2
• Specificity: Detects Human IL‑1 beta /IL‑1F2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Canakinumab. This product is for research use only.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: IL1B
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (Hu110) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

 Canakinumab is a biosimilar directed against human IL-1 beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB10349)(1)

Publication using MAB10349

• Cordeiro-Santanach, A;Morales, F;Parquet, MDC;Uaesoontrachoon, K;Rowsell, J;Warford, J;Wu, W;Elustondo, P;Hoffman, EP;Nagaraju, K; The effect of IL-1? inhibitor canakinumab (Ilaris®) on IL-6 production in human skeletal muscle cells PloS one 2025-03-06 [PMID: 40048444] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL1B