IL-1 beta/IL-1F2 Antibody (B122) [Unconjugated]

Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB4012). The ND50 is typically 0.3‑1.5 µg/mL in the presence of concanavalin A (1.25 µg/mL).

• Reactivity: Mu
• Applications: WB, IP, B/N
• Clone: B122
• Clonality: Monoclonal
• Host: Hamster
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody (B122) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-1 beta
  Accession # P10749
• Specificity: Detects mouse IL-1 beta /IL-1 F2 in direct ELISAs and Western blots. In Western blots, shows 100% cross-reactivity with recombinant rat (rr) IL‑1 beta and rcrIL‑1 beta, 15%‑35 % with rhIL‑1 beta and rpIL‑1 beta, and no cross-reactivity with rmIL-1 alpha, rmIL-18, or rhIL-1 beta rp.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Hamster
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunoprecipitation
  • Neutralization 0.3-1.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (B122) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB4012)(6)

Publications using MAB4012

• Mycobacterium tuberculosis Limits Host Glycolysis and IL-1&amp;beta by Restriction of PFK-M via MicroRNA-21
• YY Kong, GQ Li, WJ Zhang, X Hua, CC Zhou, TY Xu, ZY Li, P Wang, CY Miao Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice Acta Pharmacol. Sin., 2019;0(0):. 2019 [PMID: 30833708] (IHC-Fr, Mouse)
• DJ Li, H Fu, J Tong, YH Li, LF Qu, P Wang, FM Shen Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress Redox Biol, 2017;15(0):22-33. 2017 [PMID: 29197233] (IHC, Mouse)
• MD Maler, PJ Nielsen, N Stichling, I Cohen, Z Ruzsics, C Wood, P Engelhard, M Suomalaine, I Gyory, M Huber, J Müller-Que, WWA Schamel, S Gordon, T Jakob, SF Martin, W Jahnen-Dec, UF Greber, MA Freudenber, G Fejer Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages MBio, 2017;8(4):. 2017 [PMID: 28765216] (Western Blot, Mouse)
• Botelho F, Nikota J, Bauer C, Morissette M, Iwakura Y, Kolbeck R, Finch D, Humbles A, Stampfli M Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1alpha-dependent in mice. Respir Res, 2012;13(0):81. 2012 [PMID: 22992200] (Neutralization, Mouse)
• Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. J. Immunol., 2011;187(9):4835-43. 2011 [PMID: 21930960] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P10749()