IL-1 beta/IL-1F2 Antibody (77724) [Unconjugated]

Recombinant PorcineIL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant PorcineIL‑1 beta /IL‑1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL.

• Reactivity: Po
• Applications: WB, B/N, ELISA(Cap)
• Clone: 77724
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-1 beta/IL-1F2 Antibody (77724) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant porcine IL‑1 beta /IL-1F2
  Ala115-Pro267
  Accession # P26889.
• Specificity: Detects porcine IL-1 beta in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) IL‑1 beta and recombinant mouse (rm) IL‑1 beta is observed and no cross-reactivity with rhIL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha or recombinant rat IL-1 alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 4-15 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Porcine IL-1beta /IL-1F2 Biotinylated Antibody (Catalog number BAF681)
  Standard: Recombinant Porcine IL-1beta /IL-1F2 (Catalog number 681-PI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (77724) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta.

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. Huether, M. et al. (1993) Gene 129:285.
6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB6811)(5)

Publications using MAB6811

• Park H, Liu G, Liu Q, Zhou Y Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses, 0;10(8):. 0 [PMID: 30096906] (ELISA Development, Porcine)
• Park H, Liu G, Thulasi Raman S, Landreth S, Liu Q, Zhou Y NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC Ubiquitination. J Virol, 0;92(8):. 0 [PMID: 29386291] (ELISA Development, Porcine)
• HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination J. Virol., 2018;0(0):. 2018 [PMID: 29386291] (ELISA Capture, Porcine)
• Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate. J. Virol., 2006;80(21):10514-21. 2006 [PMID: 17041222] (Western Blot, Porcine)
• Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006;69(5):391-5. 2006 [PMID: 16604540] (Luminex Development, Porcine)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P26889.()