IL-1 beta/IL-1F2 Antibody (6E10)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-Fr, IHC-P, S-ELISA
Clone
6E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

IL-1 beta/IL-1F2 Antibody (6E10) Summary

Immunogen
Recombinant beta human Interleukin-1
Localization
Secreted
Specificity
This reacts with three different and spares determinants on Interleukin-1 beta molecule. It does not inhibit the biological activity of Interleukin-1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IL1B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
Application Notes
Detection of IL-1, beta in two-site immunoassay. We recommend using mixture of NB120-8319 and NB100-73053 for coating and NB100-73054 for labeling. For IHC on frozen and parafin embedded sections, use formalin with 2.5% glutaraldehyde as a fixative.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for IL-1 beta/IL-1F2 Antibody (6E10)

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional IL-1 beta/IL-1F2 Products

Bioinformatics Tool for IL-1 beta/IL-1F2 Antibody (NB120-8319)

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Diseases for IL-1 beta/IL-1F2 Antibody (NB120-8319)

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez