IL-1 beta/IL-1F2 Antibody (608714) [Unconjugated]

IL-1 beta /IL-1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB33401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Eq
• Applications: ICC/IF
• Clone: 608714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody (608714) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant equine IL-1 beta /IL-1F2
  Ala116-Ala268
  Accession # Q28286
• Specificity: Detects equine IL-1 beta /IL-1F2 in direct ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant canine, guinea pig, mouse, rat, and porcine IL‑1 beta is observed and 10‑50% cross‑reactivity with recombinant feline, human, rabbit, and rhesus IL‑1 beta is observed. Approximately 20% cross‑reactivity with recombinant mouse (rm) IL‑36 alpha and no cross‑reactivity with recombinant human (rh) IL‑36 alpha, rhIL‑36 gamma, rhIL‑1F10, rhIL‑36Ra, rmIL‑1Ra, rmIL‑18, or rmIL‑36 beta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 20-30 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (608714) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65%-75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta .

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
6. Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
8. Kato, H. et al. (1996) Gene 177:11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IL-1 beta/IL-1F2 Antibody (MAB33401) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Equine.

reviewed by: Verified Customer ICC Equine 03/12/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: Peripheral blood mononuclear cells (PBMCs)
• Species: Equine
• Lot: 608714

Comments

• Comments: IL‑1 beta was detected in immersion fixed equine PBMCs at 25 ug/mL for 3 hours at room temperature.

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • Q28286()