IL-1 beta/IL-1F2 Antibody (38139) [Unconjugated]

Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501). The ND50 is typically 10-30 µg/mL.

• Reactivity: Rt
• Applications: B/N, ICC/IF
• Clone: 38139
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-1 beta/IL-1F2 Antibody (38139) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat IL‑1 beta /IL‑1F2
  Val117-Ser268
  Accession # Q63264
• Specificity: Detects rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL-1 alpha , recombinant mouse IL-1 alpha , and recombinant rat IL-1 alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 10-30 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (38139) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB501)(7)

Publications using MAB501

• Seon Hee Oh, Hyun Young Lee, Young Joon Ki, Sang Hun Kim, Kyung Joon Lim, Ki Tae Jung Gabexate mesilate ameliorates the neuropathic pain in a rat model by inhibition of proinflammatory cytokines and nitric oxide pathway via suppression of nuclear factor-kappa B The Korean Journal of Pain 1/1/2020 [PMID: 31888315]
• S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury PLoS ONE, 2019-09-12;14(9):e0222354. 2019-09-12 [PMID: 31513644] (Fluorescense Microscopy, Rat)
• ME Matzkin, P Valchi, E Riviere, SP Rossi, YE Tavalieri, MMM de Toro, A Mayerhofer, A Bartke, RS Calandra, MB Frungieri Aging in the Syrian hamster testis: Inflammatory-oxidative status and the impact of photoperiod Exp. Gerontol., 2019-07-02;0(0):110649. 2019-07-02 [PMID: 31276778] (Western Blot, Hamster)
• Y Chen, Q Zhao, Q Chen, Y Zhang, B Shao, Y Jin, J Wu Melatonin attenuated inflammatory reaction by inhibiting the activation of p38 and NF??B in taurocholate?induced acute pancreatitis Mol Med Rep, 2018-02-16;0(0):. 2/16/2018 [PMID: 29484391]
• Joyal JS, Sitaras N, Binet F, Rivera JC, Stahl A, Zaniolo K, Shao Z, Polosa A, Zhu T, Hamel D, Djavari M, Kunik D, Honore JC, Picard E, Zabeida A, Varma DR, Hickson G, Mancini J, Klagsbrun M, Costantino S, Beausejour C, Lachapelle P, Smith LE, Chemtob S, Sapieha P Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A. Blood, 2011-02-25;117(22):6024-35. 2011-02-25 [PMID: 21355092] (Western Blot, Mouse)
• Morelli AE, Zahorchak AF, Larregina AT Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation. Blood, 2001-09-01;98(5):1512-23. 2001-09-01 [PMID: 11520802] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • Q63264()