IL-1 beta/IL-1F2 Antibody (38123) [Unconjugated]

Plantaris protein content results for interleukin-1 beta (IL-1 beta ) (A). Data were normalized to CON. Values are mean ± SEM (CON: n = 4; SNL: n = 4; 100CUR: n = 4; 50CMO: n = 4). Φ p < 0.050 vs. ipsilateral leg. The representative blot images display IL-1 beta and alpha -tubulin for CON, SNL, 100CUR, and 50CMO groups (B). MW, molecular weight (kDa). Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1467-3045/46/11/742), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Rt
• Applications: WB
• Clone: 38123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-1 beta/IL-1F2 Antibody (38123) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat IL-1 beta
  Val117-Ser268
  Accession # Q63264
• Specificity: Detects rat IL-1 beta in direct ELISAs and Western blots. In these formats, this antibody shows approximately 100% cross‑reactivity with recombinant mouse (rm) IL-1 beta and rcrIL-1 beta and no cross-reactivity with rfIL‑1 beta , rhIL-1 beta , or rrmIL-1 beta .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (38123) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB5011)(6)

Publications using MAB5011

• B Liu, J Xu, L Lu, L Gao, S Zhu, Y Sui, T Cao, T Yang Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis Cell Death & Disease, 2023-02-03;14(2):82. 2023-02-03 [PMID: 36737598] (Western Blot, Rat)
• Bo Lu, Jun Xie, Deyu Fu, Xiaozhe Chen, Mingyi Zhao, Mingtai Gui et al. Huoxue Qianyang Qutan recipe attenuates cardiac fibrosis by inhibiting the NLRP3 inflammasome signalling pathway in obese hypertensive rats Pharmaceutical Biology 8/7/2021 [PMID: 34362291]
• HC Xiang, LX Lin, XF Hu, H Zhu, HP Li, RY Zhang, L Hu, WT Liu, YL Zhao, Y Shu, HL Pan, M Li AMPK activation attenuates inflammatory pain through inhibiting NF-?B activation and IL-1? expression J Neuroinflammation, 2019-02-12;16(1):34. 2019-02-12 [PMID: 30755236] (Western Blot, Mouse)
• HH Szeto, S Liu, Y Soong, SV Seshan, L Cohen-Goul, V Manichev, LC Feldman, T Gustafsson Mitochondria Protection after Acute Ischemia Prevents Prolonged Upregulation of IL-1? and IL-18 and Arrests CKD J. Am. Soc. Nephrol., 2016-11-23;28(5):1437-1449. 2016-11-23 [PMID: 27881606] (Western Blot, Rat)
• Lin-Lin Kang Cinnamaldehyde and allopurinol reduce fructose-induced cardiac inflammation and fibrosis by attenuating CD36-mediated TLR4/6-IRAK4/1 signaling to suppress NLRP3 inflammasome activation Sci Rep, 2016;6(0):27460. 2016 [PMID: 27270216] (Western Blot, Rat)
• Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012-05-23;13(0):50. 2012-05-23 [PMID: 22621398] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • Q63264()