IL-1 beta/IL-1F2 Antibody (30311) [Unconjugated]

Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: 30311
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-1 beta/IL-1F2 Antibody (30311) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-1 beta /IL-1F2
  Val118-Ser269
  Accession # P10749
• Specificity: Detects mouse IL-1 beta /IL-1F2 in ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 1-5 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Mouse IL-1beta /IL-1F2 Biotinylated Antibody (Catalog number BAF401)
  Standard: Recombinant Mouse IL-1beta /IL-1F2 (Catalog number 401-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (30311) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB401)(33)

Publications using MAB401

• SY Cai, M Ge, A Mennone, R Hoque, X Ouyang, JL Boyer Inflammasome is activated in the liver of cholestatic patients and aggravates hepatic injury in bile duct ligated mouse Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31887435] (Bioassay, Human)
• DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway Mol Med Rep, 2019;0(0):. 2019 [PMID: 31746359] (ELISA Development, Mouse)
• Z Pang, R Raudonis, C McCormick, Z Cheng Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection Infect. Immun., 2019;0(0):. 2019 [PMID: 31611276] (ELISA Capture, Mouse)
• OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation Oxid Med Cell Longev, 2019;2019(0):6018180. 2019 [PMID: 31093317] (Western Blot, Mouse)
• A Polykratis, A Martens, RO Eren, Y Shirasaki, M Yamagishi, Y Yamaguchi, S Uemura, M Miura, B Holzmann, G Kollias, M Armaka, G van Loo, M Pasparakis A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain Nat. Cell Biol., 2019;0(0):. 2019 [PMID: 31086261] (Bioassay, Mouse)
• R Kolb, P Kluz, ZW Tan, N Borcherdin, N Bormann, A Vishwakarm, L Balcziak, P Zhu, BS Davies, F Gourronc, LZ Liu, X Ge, BH Jiang, K Gibson-Cor, A Klingelhut, NS Tan, Y Zhu, FS Sutterwala, X Shen, W Zhang Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4 Oncogene, 2018;0(0):. 2018 [PMID: 30518876] (ELISA Development (Capture), Mouse)
• Z Zhong, S Liang, E Sanchez-Lo, F He, S Shalapour, XJ Lin, J Wong, S Ding, E Seki, B Schnabl, AL Hevener, HB Greenberg, T Kisseleva, M Karin New mitochondrial DNA synthesis enables NLRP3 inflammasome activation Nature, 2018;0(0):. 2018 [PMID: 30046112] (Mouse)
• AK Mehta, T Doherty, D Broide, M Croft TNF Family Member LIGHT Acts with IL-1? and TGF-? to Promote Airway Remodeling During Rhinovirus Infection Allergy, 2018;0(0):. 2018 [PMID: 29315623] (Neutralization, Mouse)
• Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 29018150] (ELISA Development (Capture), Mouse)
• Y Oh, YS Kwon, BD Jung Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice Int J Med Sci, 2017;14(8):729-734. 2017 [PMID: 28824307] (ELISA Development (Capture), Mouse)
• L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis Biosci. Rep., 2017;0(0):. 2017 [PMID: 28760844] (ELISA Development (Capture), Mouse)
• SY Nam, KY Kim, MH Kim, JB Jang, SY Rah, HJ Chae, JM Lee, HM Kim, HJ Jeong Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan Pharm Biol, 2017;55(1):1856-1862. 2017 [PMID: 28614972] (ELISA Development (Capture), Mouse)
• P Dhar, GZ Ng, EM Dunne, P Sutton Mucin 1 protects against severe Streptococcus pneumoniae infection Virulence, 2017;0(0):0. 2017 [PMID: 28605238] (ELISA Development (Capture), Mouse)
• C Dempsey Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice Brain Behav. Immun, 2016;0(0):. 2016 [PMID: 28003153] (ELISA Development (Capture), Mouse)
• Yoonsook Kim Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation Pharmacogn Mag, 2016;12(48):295-301. 2016 [PMID: 27867272] (ELISA Development (Capture), Mouse)
• Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites. Proc Natl Acad Sci U S A, 2015;112(22):E2891-9. 2015 [PMID: 26038565] (ELISA Development (Capture), Mouse)
• Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. J Immunol, 2015;194(1):334-48. 2015 [PMID: 25422508] (ELISA Development, Mouse)
• Ingham V, Williams A, Bate C Glimepiride reduces CD14 expression and cytokine secretion from macrophages. J Neuroinflammation, 2014;11(0):115. 2014 [PMID: 24952384] (ELISA Development (Capture), Mouse)
• Bakker P, Butter L, Claessen N, Teske G, Sutterwala F, Florquin S, Leemans J A tissue-specific role for Nlrp3 in tubular epithelial repair after renal ischemia/reperfusion. Am J Pathol, 2014;184(7):2013-22. 2014 [PMID: 24823805] (Immunoprecipitation, Mouse)
• Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth. J Immunol, 2014;192(9):4153-63. 2014 [PMID: 24659690] (Neutralization, Mouse)
• Junkins R, MacNeil A, Wu Z, McCormick C, Lin T Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol, 2013;190(10):5178-86. 2013 [PMID: 23589609] (ELISA Development (Capture), Mouse)
• Eisenbarth, Stephani, Williams, Adam, Colegio, Oscar R, Meng, Hailong, Strowig, Till, Rongvaux, Anthony, Henao-Mejia, Jorge, Thaiss, Christop, Joly, Sophie, Gonzalez, David G, Xu, Lan, Zenewicz, Lauren A, Haberman, Ann M, Elinav, Eran, Kleinstein, Steven H, Sutterwala, Fayyaz S, Flavell, Richard NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells. Nature, 2012;484(7395):510-3. 2012 [PMID: 22538615] (Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012;58(2):169-77. 2012 [PMID: 22277799] (Luminex Development, Mouse)
• Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009;183(9):5799-806. 2009 [PMID: 19812208] (ELISA Development, Mouse)
• Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages. Eur. J. Immunol., 2009;39(3):820-5. 2009 [PMID: 19189309] (Western Blot, Mouse)
• Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8. J. Exp. Med., 2008;205(9):1967-73. 2008 [PMID: 18725521] (Western Blot, Mouse)
• Smith SE, Li J, Garbett K, Mirnics K, Patterson PH Maternal immune activation alters fetal brain development through interleukin-6. J. Neurosci., 2007;27(40):10695-702. 2007 [PMID: 17913903] (Neutralization, Mouse)
• Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor. Eur. J. Pharmacol., 2007;573(1):221-9. 2007 [PMID: 17669394] (ELISA Development, Mouse)
• Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice. Inflamm. Res., 2006;55(11):457-64. 2006 [PMID: 17122962] (ELISA Development, Mouse)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006;177(3):1967-74. 2006 [PMID: 16849510] (ELISA Development, Mouse)
• Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide. J. Invest. Dermatol., 2006;126(8):1792-803. 2006 [PMID: 16628194] (IHC-Fr, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005;302(1):172-81. 2005 [PMID: 16023134] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2004;231(1):103-11. 2004 [PMID: 15919375] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P10749()