IL-1 beta/IL-1F2 Antibody (166926) [Unconjugated]

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4011) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Pleural cells are involved in bleomycin-induced inflammation. a Cell recruitment in PLF was assessed by total cell count at D3, D14 and D21. Results are expressed as mean ± SEM. b Inflammation was estimated by differential count in PLF after May-Grünwald Giemsa staining. Results are expressed as percentages of total cells. n = 4 for NaCl groups, n = 6 for BLM groups at D3 and D21, n = 6 for NaCl and n = 8 for BLM at D14. c, d Caspase-1 activation was assessed at D14 by c western blot in whole lung extracts (n = 3 mice/group) or by d specific caspase-1 FLICA analysis on lung section from NaCl or BLM injected mice (Representative images are shown. Active caspase-1: green, DAPI: blue, dotted lines represent the pleura, scale bars: 50 μm). e, f Met5A cells were treated with BLM (100 μM) and e activation of caspase-1 was followed (western blot; left, densitometric analysis; right). f active IL-1 beta was assessed in the supernatant of Met5A cells after 24 h of culture. Representative results from 3 independent experiments. HSC70 serves as a loading control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-016-0475-8), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pleural cells are involved in bleomycin-induced inflammation. a Cell recruitment in PLF was assessed by total cell count at D3, D14 and D21. Results are expressed as mean ± SEM. b Inflammation was estimated by differential count in PLF after May-Grünwald Giemsa staining. Results are expressed as percentages of total cells. n = 4 for NaCl groups, n = 6 for BLM groups at D3 and D21, n = 6 for NaCl and n = 8 for BLM at D14. c, d Caspase-1 activation was assessed at D14 by c western blot in whole lung extracts (n = 3 mice/group) or by d specific caspase-1 FLICA analysis on lung section from NaCl or BLM injected mice (Representative images are shown. Active caspase-1: green, DAPI: blue, dotted lines represent the pleura, scale bars: 50 μm). e, f Met5A cells were treated with BLM (100 μM) and e activation of caspase-1 was followed (western blot; left, densitometric analysis; right). f active IL-1 beta was assessed in the supernatant of Met5A cells after 24 h of culture. Representative results from 3 independent experiments. HSC70 serves as a loading control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27894300), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clone: 166926
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-1 beta/IL-1F2 Antibody (166926) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-1 beta
  Val118-Ser269
  Accession # P10749
• Specificity: Detects mouse IL-1 beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-100% cross‑reactivity with human, rat, and cotton rat IL-1 beta is obseved and no cross-reactivity with recombinant porcine IL-1 beta , recombinant mouse (rm) IL-1 alpha , or rmIL-18 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (166926) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB4011)(11)

Publications using MAB4011

• Debabrata Chowdhury, Jason C. Gardner, Abhijit Satpati, Suba Nookala, Santhosh Mukundan, Aleksey Porollo et al. Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity Frontiers in Immunology 11/12/2021 [PMID: 34867993]
• Nardhy Gomez-Lopez, Roberto Romero, Valeria Garcia-Flores, Yaozhu Leng, Derek Miller, Sonia S Hassan et al. Inhibition of the NLRP3 inflammasome can prevent sterile intra-amniotic inflammation, preterm labor/birth, and adverse neonatal outcomes† Biology of Reproduction 5/1/2019 [PMID: 30596885]
• Jonathan Faro, Roberto Romero, George Schwenkel, Valeria Garcia-Flores, Marcia Arenas-Hernandez, Yaozhu Leng et al. Intra-amniotic inflammation induces preterm birth by activating the NLRP3 inflammasome† Biology of Reproduction 5/1/2019 [PMID: 30590393]
• Jonathan Faro, Roberto Romero, George Schwenkel, Valeria Garcia-Flores, Marcia Arenas-Hernandez, Yaozhu Leng et al. Intra-amniotic inflammation induces preterm birth by activating the NLRP3 inflammasome† Biology of Reproduction 2019-05-01 [PMID: 30590393] (Western Blot, Mouse)
• Pleural inhibition of the caspase-1/IL-1? pathway diminishes profibrotic lung toxicity of bleomycin Respir. Res, 2016-11-29;17(1):162. 2016-11-29 [PMID: 27894300] (Western Blot, Human)
• K Kataoka, H Matsumoto, H Kaneko, S Notomi, K Takeuchi, J H Sweigard et al. Macrophage- and RIP3-dependent inflammasome activation exacerbates retinal detachment-induced photoreceptor cell death Cell Death & Disease 4/23/2015 [PMID: 25906154]
• Akiyama T, Miyamoto Y, Yoshimura K, Yamada A, Takami M, Suzawa T, Hoshino M, Imamura T, Akiyama C, Yasuhara R, Mishima K, Maruyama T, Kohda C, Tanaka K, Potempa J, Yasuda H, Baba K, Kamijo R Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor-alpha and interleukin-1beta but suppresses that by interleukin-17A: importance of proteolytic degradation of osteoprotegerin by lysine gingipain. J Biol Chem, 2014-04-22;289(22):15621-30. 2014-04-22 [PMID: 24755218] (Western Blot, Bacteria - Porphyromonas gingivalis)
• Shin , Jin Na, Fattah , Elmoataz, Bhattacharya , Abhisek, Ko , Soyoung, Eissa , N Tony Inflammasome activation by altered proteostasis. J Biol Chem, 2013-10-31;288(50):35886-95. 2013-10-31 [PMID: 24178293] (Western Blot, Mouse)
• Bruchard M, Mignot G, Derangere V, Chalmin F, Chevriaux A, Vegran F, Boireau W, Simon B, Ryffel B, Connat J, Kanellopoulos J, Martin F, Rebe C, Apetoh L, Ghiringhelli F Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth. Nat Med, 2012-12-02;19(1):57-64. 2012-12-02 [PMID: 23202296] (Immunoprecipitation, Western Blot, Mouse)
• Aisling Dunne, Pádraig J. Ross, Eva Pospisilova, Jiri Masin, Aoife Meaney, Caroline E. Sutton et al. Inflammasome Activation by Adenylate Cyclase Toxin Directs Th17 Responses and Protection against Bordetella pertussis The Journal of Immunology 8/1/2010 [PMID: 20610650]
• Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages. J. Immunol., 2009-12-15;183(12):7890-7. 2009-12-15 [PMID: 19933859] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P10749()