Recombinant Mouse IL-1 alpha/IL-1F1 Animal-Free Protein

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SDS-Page: Recombinant Mouse IL-1 alpha/IL-1F1 Animal-Free Protein [NBP2-76212] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Mouse IL-1 alpha/IL-1F1 Animal-Free Protein [NBP2-76212] - IL-1 alpha/IL-1F1 induced proliferation of D10G41 cells.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Animal Free
Concentration
LYOPH

Order Details

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Recombinant Mouse IL-1 alpha/IL-1F1 Animal-Free Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Mouse IL-1 alpha/IL-1F1

Source: E. coli

Amino Acid Sequence: MSAPYTYQSD LRYKLMKLVR QKFVMNDSLN QTIYQDVDKH YLSTTWLNDL QQEVKFDMYA YSSGGDDSKY PVTLKISDSQ LFVSAQGEDQ PVLLKELPET PKLITGSETD LIFFWKSINS KNYFTSAAYP ELFIATKEQS RVHLARGLPS MTDFQIS

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
Specificity
IL-1 alpha/IL-1F1 is a monomer, 18.1 kDa (157 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
D10.G4.1 proliferation, >1.0 x 10^8 units/mg (ED50 acceptance criteria are <10 pg/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL1A
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse IL-1 alpha/IL-1F1 Animal-Free Protein

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL1A