Recombinant Bovine IL-1 alpha/IL-1F1 Protein

ELISA: Recombinant Bovine IL-1 alpha/IL-1F1 Protein [NBP2-62430] - Bovine Interleukin-1 alpha ELISARecombinant Bovine interleukin-1 alpha detected using Rabbit anti Bovine interleukin-1 alpha as the capture reagent and biotinylated Rabbit anti Bovine interleukin-1 alpha as the detection reagent followed by Streptavidin:HRP.

• Reactivity: Bv
• Applications: ELISA, Func
• Concentration: LYOPH

Recombinant Bovine IL-1 alpha/IL-1F1 Protein Summary

• Description: Recombinant bovine interleukin-1 alpha.
  
  

  Source:Pichia pastoris

  Amino Acid Sequence:aa 120-268 of native bovine IL-1a, UniProt P08831

• Preparation Method: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
• Source: Pichia pastoris
• Protein/Peptide Type: Recombinant Protein
• Gene: IL1A
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional
• Theoretical MW: 17.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 10% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.

Alternate Names for Recombinant Bovine IL-1 alpha/IL-1F1 Protein

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL1A
• Uniprot:
  • P08831(Bovine)