Recombinant Human IL-1 alpha/IL-1F1 Protein Summary
A single, non-glycosylated biologically active polypeptide chain corresponding to 159 residues of IL1A.
Source: E. coli
Amino Acid Sequence: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYKSSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKNYFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
IL1 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation ssay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of > 1.0 x 10^9 IU/mg.
| Protein/Peptide Type
> 97 % pure by SDS-PAGE and HPLC
Less than 1 EU/ug of endotoxin as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
> 97 % pure by SDS-PAGE and HPLC
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human IL-1 alpha/IL-1F1 Protein
- IL1 alpha
- IL-1 alpha
- interleukin 1, alpha
- interleukin-1 alpha
- preinterleukin 1 alpha
Interleukin-1 alpha (IL-1 alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1 alpha and IL-1beta binds to the same receptor and has similar identical biological properties. Among various species, the amino acid sequence of mature IL-1alpha is conserved 60 % to 70 % and human IL-1 has been found to be biologically active on murine cell lines. IL-1alpha recently started to find effective application in cosmetic and dermatological formulations, which allow to significantly harmonizing derma architecture.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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