Mouse IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

ELISA: Mouse IL-1 alpha/IL-1F1 ELISA Kit [NBP1-92673]

Product Discontinued

Mouse IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) Summary

• Description: The mouse IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-1alph
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-1 alpha positive serum. There was no crossreactivity detected.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 4.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: 75%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL1A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-1 alpha/IL-1F1 ELISA Kit (NBP1-92673). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL1A