IL-1 alpha/IL-1F1 Antibody

Images

 
Western Blot: IL-1 alpha/IL-1F1 Antibody [NBP2-16939] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: IL-1 alpha/IL-1F1 Antibody [NBP2-16939] - H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 alpha protein stained by IL1 alpha antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: IL-1 alpha/IL-1F1 Antibody [NBP2-16939] - Analysis of paraffin-embedded Colon CA, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.73 mg/ml

Order Details

IL-1 alpha/IL-1F1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IL1 alpha. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (89%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.73 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-1 alpha/IL-1F1 Antibody

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for IL-1 alpha/IL-1F1 Antibody (NBP2-16939)

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Bioinformatics

Gene Symbol IL1A
Entrez
Uniprot