IL-1 alpha/IL-1F1 Antibody [Unconjugated]

Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Polyclonal Antibody (Catalog # AB-400-NA). The ND50 is typically 0.04‑0.2 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 alpha/IL-1F1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑1 alpha /IL‑1F1
  ser6-ser161
  Accession # Q62161
• Specificity: Detects IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat IL-1 alpha is observed and less than 2% cross-reactivity with recombinant human (rh) IL-1 alpha is observed. Neutralizes the biological activity of recombinant mouse (rm) IL-1 alpha , but will not neutralize the biological activity of rhIL-1 alpha , rhIL-1 beta , or rmIL-1 beta .
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1A
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.04-0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody [Unconjugated]

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta , that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta , designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 alpha/IL-1F1 Antibody (AB-400-NA)(7)

Publications using AB-400-NA

• Shigeyuki Tamiya, Eisuke Yoshikawa, Monami Ogura, Etsushi Kuroda, Koichiro Suzuki, Yasuo Yoshioka Neutrophil-Mediated Lung Injury Both via TLR2-Dependent Production of IL-1 alpha and IL-12 p40, and TLR2-Independent CARDS Toxin after Mycoplasma pneumoniae Infection in Mice Microbiology Spectrum 2021-12-23 [PMID: 34937175] (In vivo assay, Mouse)
• Shigeyuki Tamiya, Eisuke Yoshikawa, Monami Ogura, Etsushi Kuroda, Koichiro Suzuki, Yasuo Yoshioka Neutrophil-Mediated Lung Injury Both via TLR2-Dependent Production of IL-1 alpha and IL-12 p40, and TLR2-Independent CARDS Toxin after Mycoplasma pneumoniae Infection in Mice Microbiology Spectrum 12/23/2021 [PMID: 34937175]
• HN Kim, J Xiong, RS MacLeod, S Iyer, Y Fujiwara, KM Cawley, L Han, Y He, JD Thostenson, E Ferreira, RL Jilka, D Zhou, M Almeida, CA O'Brien Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence JCI Insight, 2020-10-02;0(0):. 2020-10-02 [PMID: 32870816] (Neutralization, Mouse)
• Qingwen Zhang, Jinxia Sun, Yuli Wang, Weigang He, Lixin Wang, Yuejuan Zheng et al. Antimycobacterial and Anti-inflammatory Mechanisms of Baicalin via Induced Autophagy in Macrophages Infected with Mycobacterium tuberculosis Frontiers in Microbiology 11/2/2017 [PMID: 29163427]
• C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism Sci Rep, 2016-01-14;6(0):19404. 2016-01-14 [PMID: 26762853] (Neutralization, Mouse)
• Chan J, Atianand M, Jiang Z, Carpenter S, Aiello D, Elling R, Fitzgerald K, Caffrey D Cutting Edge: A Natural Antisense Transcript, AS-IL1alpha, Controls Inducible Transcription of the Proinflammatory Cytokine IL-1alpha. J Immunol, 2015-07-15;195(4):1359-63. 2015-07-15 [PMID: 26179904] (Western Blot, Mouse)
• Banks WA, Farr SA, La Scola ME Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum. J. Pharmacol. Exp. Ther., 2001-11-01;299(2):536-41. 2001-11-01 [PMID: 11602664] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL1A
• Uniprot:
  • Q62161()