IL-1 alpha/IL-1F1 Antibody (Hu146) [Unconjugated]

Human peripheral blood mononuclear cells were (A) treated overnight with 1 µg/mL LPS or (B) untreated, then stained with Human IL-1a/IL-1F1 (Bermekimab) Monoclonal Antibody (Catalog # MAB10568, filled histogram) or unstained (open histogram), followed by Anti-Human IgG Allophycoerythrin-conjugated Secondary Antibody (F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

• Reactivity: Hu
• Applications: Flow
• Clone: Hu146
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

IL-1 alpha/IL-1F1 Antibody (Hu146) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: Human IL‑1 alpha /IL‑1F1
• Specificity: Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Bermekimab. This product is for research use only.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody (Hu146) [Unconjugated]

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

Research grade Bermekimab is a biosimilar that binds and blocks binds to IL-1 alpha/IL-1F1. This product can be used in flow cytometry or various assay formats to block or measure IL-1 alpha/IL-1F1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.