IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated]

Images

 
Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more
IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200) for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap), ICC/IF
Clone
4414
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-1 alpha /IL-1F1
Ser113-Ala271
Accession # P01583
Specificity
Detects human IL-1 alpha /IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta , -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IL1A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.05-0.15 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human IL-1 alpha/IL-1F1 Antibody (Catalog # MAB200)
ELISA Detection: Human IL-1 alpha/IL-1F1 Biotinylated Antibody (Catalog # BAF200)
Standard: Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA)
Publications
Read Publications using
MAB200 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated]

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts, and glia (oligodendroglia, astrocytes, and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha . The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 alpha/IL-1F1 Antibody (MAB200)(30)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 11 applications: ELISA Development, ELISA Microarray Development, Electrochemiluminescent Assay, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Luminex Development, Neutralization, Western Blot.


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ELISA Development
(2)
ELISA Microarray Development
(1)
Electrochemiluminescent Assay
(2)
Flow Cytometry
(1)
ICC
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Luminex Development
(3)
Neutralization
(6)
Western Blot
(1)
All Applications
Filter By Species
Human
(21)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using MAB200 Applications Species
ES Lee, S Kim, SW Lee, J Jung, SH Lee, HW Na, HJ Kim, YD Hong, WS Park, TG Lee, DG Jo, SH Kim Molecule-Resolved Visualization of Particulate Matter on Human Skin Using Multimodal Nonlinear Optical Imaging International Journal of Molecular Sciences, 2021-05-14;22(10):. 2021-05-14 [PMID: 34069002] (IHC, Human) IHC Human
Praveen Gajawada, Ayse Cetinkaya, Susanne von Gerlach, Natalia Kubin, Heiko Burger, Michael Näbauer et al. Myocardial Accumulations of Reg3A, Reg3 gamma and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis International Journal of Molecular Sciences 4/16/2021 [PMID: 33923774]
S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare COX2 regulates senescence secretome composition and senescence surveillance through PGE2 Cell Reports, 2021-03-16;34(11):108860. 2021-03-16 [PMID: 33730589] (ICC, Western Blot, Human) ICC, Western Blot Human
Talal Salti, Khaled Khazim, Rami Haddad, Salvatore Campisi-Pinto, Gil Bar-Sela, Idan Cohen Glucose Induces IL-1 alpha -Dependent Inflammation and Extracellular Matrix Proteins Expression and Deposition in Renal Tubular Epithelial Cells in Diabetic Kidney Disease Frontiers in Immunology 7/7/2020 [PMID: 32733443]
BH Clausen, M Wirenfeldt, SS Høgedal, LH Frich, HH Nielsen, HD Schrøder, K Østergaard, B Finsen, BW Kristensen, KL Lambertsen Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke Acta Neuropathol Commun, 2020-06-05;8(1):81. 2020-06-05 [PMID: 32503645] (IHC, Human) IHC Human
Christophe Glorieux, Peng Huang Regulation of CD137 expression through K-Ras signaling in pancreatic cancer cells Cancer Communications 12/1/2019 [PMID: 31288851]
Mirella Georgouli, Cecilia Herraiz, Eva Crosas-Molist, Bruce Fanshawe, Oscar Maiques, Anna Perdrix et al. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment Cell 2/7/2019 [PMID: 30712866]
X Sun, B Shi, H Zheng, L Min, J Yang, X Li, X Liao, W Huang, M Zhang, S Xu, Z Zhu, H Cui, X Liu Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway Cell Death Dis, 2018-02-15;9(3):260. 2/15/2018 [PMID: 29449532]
CM Henry, SJ Martin Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation Mol. Cell, 2017-02-16;65(4):715-729.e5. 2017-02-16 [PMID: 28212752] (ICC, Human) ICC Human
Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling. PLoS ONE, 2016-03-31;0(0):1932-6203. 2016-03-31 [PMID: 27032103] (IHC-Fr, Human) IHC-Fr Human
Show All 30 Publications.

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Bioinformatics

Gene Symbol IL1A
Uniprot