IKK epsilon/IKBKE Antibody [Unconjugated]

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IKKe Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IKKe at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IKKe was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Goat Anti-Human IKKe Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IKK epsilon/IKBKE Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IKK epsilon
  Met1-Val716
  Accession # Q14164
• Specificity: Detects human IKK epsilon in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IKBKE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 10-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IKK epsilon/IKBKE Antibody [Unconjugated]

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase epsilon
• IKBKE
• IKK epsilon
• IKK-E
• IKKEMGC125297
• IKK-epsilon
• IKKI
• IKK-I
• IKK-iMGC125295
• Inducible I kappa-B kinase
• inducible IkappaB kinase
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• inhibitor of nuclear factor kappa-B kinase subunit epsilon
• KIAA0151IKK-related kinase epsilon
• MGC125294

Background

I kappa B kinase epsilon (IKK epsilon ) is also known as IKBKE and IKKi. The classical active IKK complex, composed of IKK alpha , IKK beta , and two forms of processed IKK gamma , phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK epsilon is an IKK-related kinase with 30% structural homology to IKK alpha and beta , and is present in a subset of IKK complexes. IKK epsilon activation has been implicated in the initiation of anti-viral gene expression and in the pathogenesis of breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKBKE
• Uniprot:
  • Q14164()