Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - NB100-56513 was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. ...read more
Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56513. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram ...read more
Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Simple Western lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
Western Blot 2-5 ug/ml
Simple Western 1:12.5
Flow Cytometry 1:10-1:1000
Gel Super Shift Assays
Immunoprecipitation 2-5 ug/ 10^6 cells
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24825920)In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Gel Shift Assays reported in scientific literature (PMID 19662631)
Read Publications using NB100-56513 in the following applications:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
inhibitor of nuclear factor kappa-B kinase subunit beta
Nuclear factor NF-kappa-B inhibitor kinase beta
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.