IKK beta Antibody (10A9B6)

Images

 
Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56513. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram ...read more
Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Antibodoy was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was ...read more
Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Simple Western lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, GS, ICC/IF, IP
Clone
10A9B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

IKK beta Antibody (10A9B6) Summary

Immunogen
Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).
Specificity
An approx. 87 kDa band should be observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IKBKB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Simple Western 1:12.5
  • Flow Cytometry 1:10-1:1000
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-5 ug/ 10^6 cells
  • Flow (Intracellular)
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24825920) Use in Gel Shift Assays reported in scientific literature (PMID 19662631).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NB100-56513 in the following applications:

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IKK beta Antibody (10A9B6)

  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase 2
  • I-kappa-B-kinase beta
  • IkBKB
  • IKK beta
  • IKK2
  • IKK2FLJ38368
  • IKK-B
  • IKK-betaFLJ36218
  • IKKBFLJ40509
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of nuclear factor kappa-B kinase subunit beta
  • MGC131801
  • NFKBIKB
  • NFKBIKBFLJ33771
  • Nuclear factor NF-kappa-B inhibitor kinase beta

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IKK beta Antibody (NB100-56513)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: EMSA, ICC/IF, WB.


Filter By Application
EMSA
(1)
ICC/IF
(1)
WB
(3)
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Human
(1)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-56513 Applications Species
Paul S, Traver MK, Kashyap AK et al. T Cell Receptor Signals to NF-kB Are Transmitted by a Cytosolic p62-Bcl10-Malt1-Ikk Signalosome. Sci Signal. 2014 May 14 [PMID: 24825920] (ICC/IF, Mouse) ICC/IF Mouse
Bakkar N, Ladner K, Canan BD et al. IKKa and alternative NF-kB regulate PGC-1B to promote oxidative muscle metabolism. J Cell Biol. 2012 Feb 20 [PMID: 22351927]
Yang Gong, Rosen Daniel G, Liu Guangzhi et al. CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis. Clin Cancer Res. 2010 Aug 1 [PMID: 20505188] (WB) WB
Tare Nadine, Li Hongli, Morschauser Andrew et al. KU812 cells provide a novel in vitro model of the human IL-33/ST2L axis: functional responses and identification of signaling pathways. Exp Cell Res. 2010 Sep 10 [PMID: 20406635] (WB, Human) WB Human
Monsalve Eva, Ruiz-Garcia Almudena, Baladron Victoriano et al. Notch1 upregulates LPS-induced macrophage activation by increasing NF-kappaB activity. Eur J Immunol. 2009 Sep [PMID: 19662631] (EMSA) EMSA
Scott MJ, Billiar TR. Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes. J Biol Chem. 2008 Oct 24 [PMID: 18701460]
Lamothe B, Campos AD, Webster WK et al. The RING domain and first zinc finger of TRAF6 coordinate signaling by interleukin-1, lipopolysaccharide, and RANKL. J Biol Chem. 2008 Sep 5 [PMID: 18617513]
Vacca Alessandra, Felli Maria Pia, Palermo Rocco et al. Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia. EMBO J. 2006 Mar 8 [PMID: 16498412] (Mouse) Mouse
Fitzgerald KA, McWhirter SM, Faia KL et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat Immunol. 2003 May [PMID: 12692549] (WB)

Details:
Antibodies cited [WB (293T cells transfected with siRNA), Fig. 3a]: 1. TBK [IMG-139A] IP (293T cells transfected with siRNA), Fig. 3a.
WB

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IKK beta Antibody (NB100-56513) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56513AF405
Alexa Fluor 488 Labeled NB100-56513AF488
Alexa Fluor 647 Labeled NB100-56513AF647
Alexa Fluor 700 Labeled NB100-56513AF700
Allophycocyanin Labeled NB100-56513APC
Biotin Labeled NB100-55780
DyLight 350 Labeled NB100-56513UV
DyLight 405 Labeled NB100-56513V
DyLight 488 Labeled NB100-56513G
DyLight 550 Labeled NB100-56513R
DyLight 650 Labeled NB100-56513C
DyLight 680 Labeled NB100-56513FR
DyLight 755 Labeled NB100-56513IR
HRP Labeled NB100-56513H
PE Labeled NB100-56513PE
PerCP Labeled NB100-56513PCP

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Blogs on IKK beta.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

IKK alpha: Roles in Development, B-cell Survival and ESC Differentiation
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK1 alpha) is a serine/threonine kinase that forms a complex with IKK beta and NEMO. It plays an essential role in embryonic skin development. Mice with low levels of IKKa show an increase in...  Read full blog post.

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Bioinformatics

Gene Symbol IKBKB
Entrez
Uniprot