IGSF8/CD316 Antibody (2587G) [Unconjugated]

Western blot shows lysates of human brain (hippocampus) tissue, mouse brain (cerebellum) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat IGSF8/CD316 Monoclonal Antibody (Catalog # MAB31171) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for IGSF8/CD316 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

SHSY-5Y human neuroblastoma cell line was stained with Rabbit Anti-Human/Mouse/Rat IGSF8/CD316 Monoclonal Antibody (Catalog # MAB31171, filled histogram) or Rabbit IgG isotype control antibody (MAB1050, open histogram) followed by Anti-Rabbit IgG APC-conjugated Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Simple Western lane view shows lysates of Human Brain (Cerebellum), loaded at 0.2 mg/mL. A specific band was detected for IGSF8/CD316 at approximately 73 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat IGSF8/CD316 Monoclonal Antibody (Catalog # MAB31171). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, CyTOF-ready, Flow
• Clone: 2587G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IGSF8/CD316 Antibody (2587G) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell, HEK293-derived human IGSF8/CD316
  Arg28-Thr579
  Accession # Q969P0-1
• Specificity: Detects human IGSF8/CD316 in direct ELISAs. Detects human, mouse, and rat IGSF8/CD316 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGSF8/CD316 Antibody (2587G) [Unconjugated]

• CD316 antigen
• CD316
• CD81P3
• CD81P3LIR-D1
• EWI2
• EWI-2
• EWI2CD81 partner 3
• Glu-Trp-Ile EWI motif-containing protein 2
• IGSF8
• immunoglobulin superfamily, member 8
• KCT4
• KCT-4
• Keratinocytes-associated transmembrane protein 4
• PGRL
• PGRLimmunoglobulin superfamily member 8

Background

IGSF8 (Immunoglobulin superfamily member 8), also known as EWI-2, KCT-4, LIR-D1, and PGRL, is a 75-kDa cell surface protein belonging to the immunoglobulin superfamily (1). IGSF8 is widely expressed, with pronounced mRNA expression in the brain and protein expression on peripheral blood lymphocytes and hepatocytes where it colocalizes with CD81 (1-3). It strongly associates with tetraspanins CD9 and CD81 which may act as physical linkers to form a complex with alpha 3 beta 1 integrin that may regulate cell aggregation and motility on laminin-5 (4). Human IGSF8 is synthesized as a 613 aa protein that includes a 27 aa signal peptide, a 552 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within the ECD, human IGSF8 shares 91% and 90% aa sequence identity with mouse and rat IGSF8, respectively. IGSF8 is an inducible receptor for Heat Shock Protein A8 (HSPA8) on activated dendritic cells (5). IGSF8 can interact with alpha -Actinin to regulate T cell immune synapses and HIV viral infection (6). In human glioma patients, low IGSF8 expression correlates with shorter survival time. Studies have shown that re-expression of IGSF8 in malignant glioblastoma cell lines inhibited glioblastoma colony formation in soft agar and caused diminished cell motility and invasion (7).

1. Clark, K.L. et al. (2001) J. Immunol. 167:5115.
2. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
3. Charrin, S. et al. (2003) Biochem. J. 373:409.
4. Stipp, C.S. et al. (2003) J. Cell Biol. 163:1167.
5. Kettner, S. et al. (2007) Mol Cell Biol. 27:7718.
6. Gordón-Alonso, M. et al. (2012) J Immunol 189:689.
7. Kolesnikova, T. et al. (2009) Neoplasia.11:77.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q969P0-1()