IGSF8/CD316 Antibody (2587G) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt
• Clone: 2587G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

IGSF8/CD316 Antibody (2587G) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human IGSF8/CD316 in direct ELISAs. Detects human, mouse, and rat IGSF8/CD316 in Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGSF8/CD316 Antibody (2587G) [Alexa Fluor™ Plus 647]

• CD316 antigen
• CD316
• CD81P3
• CD81P3LIR-D1
• EWI2
• EWI-2
• EWI2CD81 partner 3
• Glu-Trp-Ile EWI motif-containing protein 2
• IGSF8
• immunoglobulin superfamily, member 8
• KCT4
• KCT-4
• Keratinocytes-associated transmembrane protein 4
• PGRL
• PGRLimmunoglobulin superfamily member 8

Background

IGSF8 (Immunoglobulin superfamily member 8), also known as EWI-2, KCT-4, LIR-D1, and PGRL, is a 75-kDa cell surface protein belonging to the immunoglobulin superfamily (1). IGSF8 is widely expressed, with pronounced mRNA expression in the brain and protein expression on peripheral blood lymphocytes and hepatocytes where it colocalizes with CD81 (1-3). It strongly associates with tetraspanins CD9 and CD81 which may act as physical linkers to form a complex with alpha 3 beta 1 integrin that may regulate cell aggregation and motility on laminin-5 (4). Human IGSF8 is synthesized as a 613 aa protein that includes a 27 aa signal peptide, a 552 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within the ECD, human IGSF8 shares 91% and 90% aa sequence identity with mouse and rat IGSF8, respectively. IGSF8 is an inducible receptor for Heat Shock Protein A8 (HSPA8) on activated dendritic cells (5). IGSF8 can interact with alpha -Actinin to regulate T cell immune synapses and HIV viral infection (6). In human glioma patients, low IGSF8 expression correlates with shorter survival time. Studies have shown that re-expression of IGSF8 in malignant glioblastoma cell lines inhibited glioblastoma colony formation in soft agar and caused diminished cell motility and invasion (7).

1. Clark, K.L. et al. (2001) J. Immunol. 167:5115.
2. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
3. Charrin, S. et al. (2003) Biochem. J. 373:409.
4. Stipp, C.S. et al. (2003) J. Cell Biol. 163:1167.
5. Kettner, S. et al. (2007) Mol Cell Biol. 27:7718.
6. Gordón-Alonso, M. et al. (2012) J Immunol 189:689.
7. Kolesnikova, T. et al. (2009) Neoplasia.11:77.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.