IGSF2/CD101 Antibody (BB27) - BSA Free

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock.

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock.

• Reactivity: Hu
• Applications: Flow, IHC, IP
• Clone: BB27
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IGSF2/CD101 Antibody (BB27) - BSA Free Summary

• Additional Information: Clone BB27 was used by HLDA to establish CD designation.
• Immunogen: Human thymic clone B12
• Specificity: NB100-65269 recognizes human CD101. CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. Clone BB27 has been reported to inhibit allogeneic T-cell responses (1). Removal of sodium azide is recommended prior to use in functional assays.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD101
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IGSF2/CD101 Antibody (BB27) - BSA Free

• CD101 antigen
• CD101 molecule
• CD101
• Cell surface glycoprotein V7
• EWI101
• EWI-101
• Glu-Trp-Ile EWI motif-containing protein 101
• IGSF2
• Leukocyte Surface Protein
• member 2
• V7

Background

CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from platelets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD101
• Uniprot:
  • Q93033(Human)