IGSF3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IGSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4788) followed by HRP-conjugated Anti-Sheep ...read more
A549 human lung carcinoma cell line was stained with Sheep Anti-Human IGSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4788, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price

IGSF3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGSF3
Gln20-Ala1125
Accession # AAI10652
Specificity
Detects human IGSF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IGSF4B, rhIGSF4C, and rhIGSF8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IGSF3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGSF3 Antibody [Unconjugated]

  • EWI-3
  • glu-Trp-Ile EWI motif-containing protein 3
  • IGSF3
  • immunoglobulin superfamily, member 3
  • member 3
  • V8

Background

IGSF3 (Immunoglobulin superfamily member 3; also EWI-3) is a 133 kDa (predicted) member of the EWI subfamily of the Ig-Superfamily of molecules. It is reported to be widely expressed, with concentration in placenta and lung. Human IGSF3 an 1194 amino acid (aa) type I transmembrane protein that contains a 19 aa signal sequence followed by an 1175 aa mature region (aa 20-1194) (SwissProt # O75054). The molecule's extracellular region is 1105 aa in length (aa 20-1124), and contains eight C2-type Ig-like domains (aa 22-1097). This region also possesses at least two utilized phosphorylation sites at Thr617and Ser625, plus an EWI motif in the second Ig-like domain. IGSF3 likely exists as a disulfide-linked homodimer. There are two potential isoform variants. One contains a 20 aa insertion after Pro406, while another shows the same insert coupled to a premature truncation after Pro833. GenBank Accession # AAI10652 is identical to the SwissProt sequence above save for a double Glu substitution for Asp1020 in the eighth Ig-like domain. Over aa 20‑1125, human IGSF3 shares 92% aa identity with mouse IGSF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGSF3
Entrez
Uniprot